Affinity chromatography in the industrial purification of plasma proteins for therapeutic use

Thierry Burnouf, Mirjana Radosevich

Research output: Contribution to journalReview article

81 Citations (Scopus)

Abstract

Affinity chromatography is a powerful technique for the purification of many proteins in human plasma. Applications cover the isolation of proteins for research purposes but also, to a large extent, for the production of therapeutic products. In industrial plasma fractionation, affinity chromatography has been found to be particularly advantageous for fine and rapid capture of plasma proteins from industrial plasma fractions pre-purified by ethanol fractionation or by ion-exchange chromatography. To date, affinity chromatography is being used in the production of various licensed therapeutic plasma products, such as the concentrates of Factor VIII, Factor IX, von Willebrand Factor, Protein C, Antithrombin III, and Factor XI. Most commonly used ligands are heparin, gelatin, murine antibodies, and, to a lesser extent, Cu2+. Possible development of the use of affinity chromatography in industrial plasma fractionation should be associated to the current development of phage display and combinatorial chemistry. Both approaches may lead to the development of tailor-made synthetic ligands that would allow implementation of protein capture technology, providing improved productivity and yield for plasma products.

Original languageEnglish
Pages (from-to)575-586
Number of pages12
JournalJournal of Biochemical and Biophysical Methods
Volume49
Issue number1-3
DOIs
Publication statusPublished - Oct 30 2001
Externally publishedYes

Keywords

  • Affinity chromatography
  • Industrial purification
  • Plasma proteins
  • Therapeutic use

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics

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