Advanced glycosylated end products restrain the osteogenic differentiation of the periodontal ligament stem cell

Zhu Ling Guo, Shan Ling Gan, Chun Yi Cao, Rao Fu, Sheng Ping Cao, Chen Xie, Jing Wei Chen, Alex Gibson, Xu Zheng, Nai chia Teng

Research output: Contribution to journalArticle


Background/purpose: Many studies have confirmed that periodontal disease interacts with diabetes. The aim of this study was to examine whether the advanced glycosylated end products (AGEs), which are generated by diabetics, have important effects on the osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Materials and methods: In this study PDLSCs were isolated from the periodontal ligaments of extracted third molar teeth. The subjects were divided into two groups, which included the normal control group (N-PDLSCs) and the AGEs-stimulating group (A-PDLSCs). Changes of receptor of AGEs (RAGE) and cumulative ROS in PDLSCs were monitored by western blot and flow cytometry, respectively. Results: In the study AGEs noticeably inhibited the osteogenic differentiation of PDLSCs, with significant lower calcification nodules detected in A-PDLSCs (P < 0.01). RAGE expression level and ROS accumulation in A-PDLSCs were clearly higher than those in N-PDLSCs (P < 0.01). Conclusion: Our conclusions were that AGEs may cause the apoptosis of stem cells, which could lead to the disorder of bone differentiation function of PDLSCs.

Original languageEnglish
JournalJournal of Dental Sciences
Publication statusPublished - Jan 1 2019



  • AGEs (advanced glycosylated end products)
  • Diabetes mellitus
  • PDLSCs (periodontal ligament stem cells)
  • Periodontal disease
  • Prevention

ASJC Scopus subject areas

  • Dentistry(all)

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