Adipocytes modulate the electrophysiology of atrial myocytes: Implications in obesity-induced atrial fibrillation

Yung Kuo Lin, Yao Chang Chen, Jenn Han Chen, Shih Ann Chen, Yi Jen Chen

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Obesity is an important risk factor for atrial fibrillation (AF). Increased epicardial adipose tissue in obesity can enhance inflammation and plays an important role in the pathophysiology of AF. However, it is not clear whether epicardial adipocytes directly modulate the electrophysiological characteristics of atrial myocytes. Wholecell patch clamp was used to record the action potentials (APs) and ionic currents in isolated rabbit left atrium (LA) myocytes incubated with and without (control) isolated adipocytes from epicardial, retrosternal, or abdominal adipose tissues, or adipocytes-conditioned supernatant for 2-4 h. Compared to control LA myocytes (n = 22), LA myocytes incubated with epicardial (n = 17), retrosternal (n = 18), or abdominal adipocytes (n = 22) had longer (80 ± 3, 109 ± 6, 109 ± 6, and 110 ± 7 ms, p\0.001) 90 % AP durations (APD90). LA myocytes incubated with epicardial adipocytes had a more-positive resting membrane potential (RMP) than control LA myocytes (-57 ± 1 mV vs. -63.4 ± 1.4 mV, p\0.05). However, LA myocytes (n = 32) incubated with supernatant had longer APD90 (93 ± 3 ms, p\0.05), but similar RMP values (-62 ± 2 mV, p[0.05) in comparison to control myocytes. Epicardial adipocyte-incubated LA myocytes had larger late sodium currents, L-type calcium currents, and transient outward potassium currents, but smaller delayed rectifier potassium and inward rectifier potassium currents than control LA myocytes. Moreover, isoproterenol (10 nM) induced a higher incidence (67 vs. 22 %, p\0.05) of triggered beats in adipocytes-incubated LA myocytes (n = 12) than in control LA myocytes (n = 9). In conclusion, adipocytes can directly modulate the electrophysiological properties and ion currents, causing higher arrhythmogenesis in LA myocytes.

Original languageEnglish
Article number293
JournalBasic Research in Cardiology
Volume107
Issue number5
DOIs
Publication statusPublished - 2012

Fingerprint

Electrophysiology
Adipocytes
Atrial Fibrillation
Muscle Cells
Heart Atria
Obesity
Potassium
Membrane Potentials
Action Potentials
Abdominal Fat
Isoproterenol
Adipose Tissue
Sodium

Keywords

  • Adipokines
  • Atrial fibrillation
  • Epicardial fat
  • Obesity

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)
  • Physiology

Cite this

Adipocytes modulate the electrophysiology of atrial myocytes : Implications in obesity-induced atrial fibrillation. / Lin, Yung Kuo; Chen, Yao Chang; Chen, Jenn Han; Chen, Shih Ann; Chen, Yi Jen.

In: Basic Research in Cardiology, Vol. 107, No. 5, 293, 2012.

Research output: Contribution to journalArticle

@article{cd15d6c03399421584fda20cd23afc21,
title = "Adipocytes modulate the electrophysiology of atrial myocytes: Implications in obesity-induced atrial fibrillation",
abstract = "Obesity is an important risk factor for atrial fibrillation (AF). Increased epicardial adipose tissue in obesity can enhance inflammation and plays an important role in the pathophysiology of AF. However, it is not clear whether epicardial adipocytes directly modulate the electrophysiological characteristics of atrial myocytes. Wholecell patch clamp was used to record the action potentials (APs) and ionic currents in isolated rabbit left atrium (LA) myocytes incubated with and without (control) isolated adipocytes from epicardial, retrosternal, or abdominal adipose tissues, or adipocytes-conditioned supernatant for 2-4 h. Compared to control LA myocytes (n = 22), LA myocytes incubated with epicardial (n = 17), retrosternal (n = 18), or abdominal adipocytes (n = 22) had longer (80 ± 3, 109 ± 6, 109 ± 6, and 110 ± 7 ms, p\0.001) 90 {\%} AP durations (APD90). LA myocytes incubated with epicardial adipocytes had a more-positive resting membrane potential (RMP) than control LA myocytes (-57 ± 1 mV vs. -63.4 ± 1.4 mV, p\0.05). However, LA myocytes (n = 32) incubated with supernatant had longer APD90 (93 ± 3 ms, p\0.05), but similar RMP values (-62 ± 2 mV, p[0.05) in comparison to control myocytes. Epicardial adipocyte-incubated LA myocytes had larger late sodium currents, L-type calcium currents, and transient outward potassium currents, but smaller delayed rectifier potassium and inward rectifier potassium currents than control LA myocytes. Moreover, isoproterenol (10 nM) induced a higher incidence (67 vs. 22 {\%}, p\0.05) of triggered beats in adipocytes-incubated LA myocytes (n = 12) than in control LA myocytes (n = 9). In conclusion, adipocytes can directly modulate the electrophysiological properties and ion currents, causing higher arrhythmogenesis in LA myocytes.",
keywords = "Adipokines, Atrial fibrillation, Epicardial fat, Obesity",
author = "Lin, {Yung Kuo} and Chen, {Yao Chang} and Chen, {Jenn Han} and Chen, {Shih Ann} and Chen, {Yi Jen}",
year = "2012",
doi = "10.1007/s00395-012-0293-1",
language = "English",
volume = "107",
journal = "Basic Research in Cardiology",
issn = "0300-8428",
publisher = "D. Steinkopff-Verlag",
number = "5",

}

TY - JOUR

T1 - Adipocytes modulate the electrophysiology of atrial myocytes

T2 - Implications in obesity-induced atrial fibrillation

AU - Lin, Yung Kuo

AU - Chen, Yao Chang

AU - Chen, Jenn Han

AU - Chen, Shih Ann

AU - Chen, Yi Jen

PY - 2012

Y1 - 2012

N2 - Obesity is an important risk factor for atrial fibrillation (AF). Increased epicardial adipose tissue in obesity can enhance inflammation and plays an important role in the pathophysiology of AF. However, it is not clear whether epicardial adipocytes directly modulate the electrophysiological characteristics of atrial myocytes. Wholecell patch clamp was used to record the action potentials (APs) and ionic currents in isolated rabbit left atrium (LA) myocytes incubated with and without (control) isolated adipocytes from epicardial, retrosternal, or abdominal adipose tissues, or adipocytes-conditioned supernatant for 2-4 h. Compared to control LA myocytes (n = 22), LA myocytes incubated with epicardial (n = 17), retrosternal (n = 18), or abdominal adipocytes (n = 22) had longer (80 ± 3, 109 ± 6, 109 ± 6, and 110 ± 7 ms, p\0.001) 90 % AP durations (APD90). LA myocytes incubated with epicardial adipocytes had a more-positive resting membrane potential (RMP) than control LA myocytes (-57 ± 1 mV vs. -63.4 ± 1.4 mV, p\0.05). However, LA myocytes (n = 32) incubated with supernatant had longer APD90 (93 ± 3 ms, p\0.05), but similar RMP values (-62 ± 2 mV, p[0.05) in comparison to control myocytes. Epicardial adipocyte-incubated LA myocytes had larger late sodium currents, L-type calcium currents, and transient outward potassium currents, but smaller delayed rectifier potassium and inward rectifier potassium currents than control LA myocytes. Moreover, isoproterenol (10 nM) induced a higher incidence (67 vs. 22 %, p\0.05) of triggered beats in adipocytes-incubated LA myocytes (n = 12) than in control LA myocytes (n = 9). In conclusion, adipocytes can directly modulate the electrophysiological properties and ion currents, causing higher arrhythmogenesis in LA myocytes.

AB - Obesity is an important risk factor for atrial fibrillation (AF). Increased epicardial adipose tissue in obesity can enhance inflammation and plays an important role in the pathophysiology of AF. However, it is not clear whether epicardial adipocytes directly modulate the electrophysiological characteristics of atrial myocytes. Wholecell patch clamp was used to record the action potentials (APs) and ionic currents in isolated rabbit left atrium (LA) myocytes incubated with and without (control) isolated adipocytes from epicardial, retrosternal, or abdominal adipose tissues, or adipocytes-conditioned supernatant for 2-4 h. Compared to control LA myocytes (n = 22), LA myocytes incubated with epicardial (n = 17), retrosternal (n = 18), or abdominal adipocytes (n = 22) had longer (80 ± 3, 109 ± 6, 109 ± 6, and 110 ± 7 ms, p\0.001) 90 % AP durations (APD90). LA myocytes incubated with epicardial adipocytes had a more-positive resting membrane potential (RMP) than control LA myocytes (-57 ± 1 mV vs. -63.4 ± 1.4 mV, p\0.05). However, LA myocytes (n = 32) incubated with supernatant had longer APD90 (93 ± 3 ms, p\0.05), but similar RMP values (-62 ± 2 mV, p[0.05) in comparison to control myocytes. Epicardial adipocyte-incubated LA myocytes had larger late sodium currents, L-type calcium currents, and transient outward potassium currents, but smaller delayed rectifier potassium and inward rectifier potassium currents than control LA myocytes. Moreover, isoproterenol (10 nM) induced a higher incidence (67 vs. 22 %, p\0.05) of triggered beats in adipocytes-incubated LA myocytes (n = 12) than in control LA myocytes (n = 9). In conclusion, adipocytes can directly modulate the electrophysiological properties and ion currents, causing higher arrhythmogenesis in LA myocytes.

KW - Adipokines

KW - Atrial fibrillation

KW - Epicardial fat

KW - Obesity

UR - http://www.scopus.com/inward/record.url?scp=84864648167&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84864648167&partnerID=8YFLogxK

U2 - 10.1007/s00395-012-0293-1

DO - 10.1007/s00395-012-0293-1

M3 - Article

C2 - 22886089

AN - SCOPUS:84864648167

VL - 107

JO - Basic Research in Cardiology

JF - Basic Research in Cardiology

SN - 0300-8428

IS - 5

M1 - 293

ER -