Adding the p16INK4a marker to the traditional 3-marker (ER/Vim/CEA) panel engenders no supplemental benefit in distinguishing between primary endocervical and endometrial adenocarcinomas in a tissue microarray study

Chih Ping Han, Ming Yung Lee, Lai Fong Kok, Alexandra Ruan, Tina S. Wu, Ya Wen Cheng, Yeu Sheng Tyan, Ching Yi Lin

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Endocervical adenocarcinomas (ECAs) and endometrial adenocarcinomas (EMAs) are malignancies that affect the uterus; however, their biologic behaviors are quite different. This distinction has clinical significance, because the appropriate therapy may depend on the site of tumor origin. In this study, we not only compare the individual expression status of 4 immunomarkers [estrogen receptor (ER), vimentin (Vim), carcinoembryonic antigen (CEA), and p16], but also evaluate whether p16 adds value to the ER/Vim/CEA panel characteristics and performance in distinguishing between primary ECA and EMA. A tissue microarray (TMA) was constructed using paraffin-embedded, formalin-fixed tissues from 38 hysterectomy specimens, including 14 ECAs and 24 EMAs. Tissue microarray sections were immunostained with 4 antibodies, by the avidin-biotin complex method for antigen visualization. The staining intensity and area extent of the immunohistochemical reactions were evaluated using the semiquantitative scoring system. The 3 markers (ER, Vim, CEA) and their respective panel expressions showed statistically significant (P<0.05) frequency differences in ECA and EMA tumors. The p16 marker also revealed a significant frequency difference (P<0.05) between ECA and EMA, but did not demonstrate any supplementary benefit to the traditional 3-marker panel. In conclusion, when histomorphologic and clinical doubt exist as to the primary site of origin, we suggest that the conventional 3-marker (ER/Vim/CEA) panel is appropriate. Ancillary p16-marker testing does not add value to the 3-marker panel in distinguishing between primary ECA and EMA. © 2009 International Society of Gynecological Pathologists.

Original languageEnglish
Pages (from-to)489-496
Number of pages8
JournalInternational Journal of Gynecological Pathology
Volume28
Issue number5
DOIs
Publication statusPublished - Sep 2009
Externally publishedYes

Fingerprint

Carcinoembryonic Antigen
Vimentin
Estrogen Receptors
Adenocarcinoma
Neoplasms
Avidin
Biotin
Hysterectomy
Paraffin
Formaldehyde
Uterus
Staining and Labeling

Keywords

  • Carcinoembryonic antigen
  • Endocervical adenocarcinomas
  • Endometrial adenocarcinomas
  • Estrogen receptor
  • Immunohistochemistry
  • p16
  • Tissue microarray
  • Vimentin

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Obstetrics and Gynaecology

Cite this

Adding the p16INK4a marker to the traditional 3-marker (ER/Vim/CEA) panel engenders no supplemental benefit in distinguishing between primary endocervical and endometrial adenocarcinomas in a tissue microarray study. / Han, Chih Ping; Lee, Ming Yung; Kok, Lai Fong; Ruan, Alexandra; Wu, Tina S.; Cheng, Ya Wen; Tyan, Yeu Sheng; Lin, Ching Yi.

In: International Journal of Gynecological Pathology, Vol. 28, No. 5, 09.2009, p. 489-496.

Research output: Contribution to journalArticle

Han, Chih Ping ; Lee, Ming Yung ; Kok, Lai Fong ; Ruan, Alexandra ; Wu, Tina S. ; Cheng, Ya Wen ; Tyan, Yeu Sheng ; Lin, Ching Yi. / Adding the p16INK4a marker to the traditional 3-marker (ER/Vim/CEA) panel engenders no supplemental benefit in distinguishing between primary endocervical and endometrial adenocarcinomas in a tissue microarray study. In: International Journal of Gynecological Pathology. 2009 ; Vol. 28, No. 5. pp. 489-496.
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abstract = "Endocervical adenocarcinomas (ECAs) and endometrial adenocarcinomas (EMAs) are malignancies that affect the uterus; however, their biologic behaviors are quite different. This distinction has clinical significance, because the appropriate therapy may depend on the site of tumor origin. In this study, we not only compare the individual expression status of 4 immunomarkers [estrogen receptor (ER), vimentin (Vim), carcinoembryonic antigen (CEA), and p16], but also evaluate whether p16 adds value to the ER/Vim/CEA panel characteristics and performance in distinguishing between primary ECA and EMA. A tissue microarray (TMA) was constructed using paraffin-embedded, formalin-fixed tissues from 38 hysterectomy specimens, including 14 ECAs and 24 EMAs. Tissue microarray sections were immunostained with 4 antibodies, by the avidin-biotin complex method for antigen visualization. The staining intensity and area extent of the immunohistochemical reactions were evaluated using the semiquantitative scoring system. The 3 markers (ER, Vim, CEA) and their respective panel expressions showed statistically significant (P<0.05) frequency differences in ECA and EMA tumors. The p16 marker also revealed a significant frequency difference (P<0.05) between ECA and EMA, but did not demonstrate any supplementary benefit to the traditional 3-marker panel. In conclusion, when histomorphologic and clinical doubt exist as to the primary site of origin, we suggest that the conventional 3-marker (ER/Vim/CEA) panel is appropriate. Ancillary p16-marker testing does not add value to the 3-marker panel in distinguishing between primary ECA and EMA. {\circledC} 2009 International Society of Gynecological Pathologists.",
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AU - Lee, Ming Yung

AU - Kok, Lai Fong

AU - Ruan, Alexandra

AU - Wu, Tina S.

AU - Cheng, Ya Wen

AU - Tyan, Yeu Sheng

AU - Lin, Ching Yi

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