ADAR1 deaminase contributes to scheduled skeletal myogenesis progression via stage-specific functions

C. L. Hsieh, H. Liu, Y. Huang, L. Kang, H. W. Chen, Y. T. Chen, Y. R. Wee, S. J. Chen, B. C M Tan

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Adenosine deaminases acting on RNA 1 (ADAR1) catalyzes cellular RNA adenosine-to-inosine editing events on structured RNA molecules. In line with this critical role, ADAR1 exhibits ubiquitous expression and is essential for embryonic development. However, regulation and developmental significance of this RNA editor in a spatiotemporal context are largely elusive. Here we unveil a novel tissue-specific role of ADAR1 in skeletal myogenesis. ADAR1 expression displayed programmed alteration that is coordinated with differentiation cues, and mediated negatively by miRNA-1/206. Coincidently, ADAR1 exerts stage-dependent functions-suppression of apoptosis at the onset of differentiation and preservation of timely myotube formation through later phase. Furthermore, the post-transcriptional aspect of its myogenic role was illustrated by the spectrum of binding RNAs, as revealed by high-throughput approach, as well as by direct regulation of myogenesis-associated targets such as dynamin 1/2 (Dnm1/2) and annexin A4. Consequently, maintenance of target gene expression profiles likely contributes to a state of cytoskeleton and membrane dynamics that is amenable to myoblast morphogenesis. Collectively, these findings uncover a critical link of ADAR1 to myogenesis, and further highlight an epigenetic mechanism by which ADAR1 and miR-1/206 interplay to control scheduled myoblast-myotube transition.

Original languageEnglish
Pages (from-to)707-719
Number of pages13
JournalCell Death and Differentiation
Volume21
Issue number5
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Adenosine Deaminase
Muscle Development
RNA
Myoblasts
Skeletal Muscle Fibers
Annexin A4
Dynamin I
Dynamin II
Inosine
Cytoskeleton
MicroRNAs
Morphogenesis
Transcriptome
Epigenomics
Adenosine
Embryonic Development
Cues
Maintenance
Apoptosis

Keywords

  • ADAR1
  • dynamins
  • miRNA-1/206
  • myogenesis
  • RIP-Seq

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

ADAR1 deaminase contributes to scheduled skeletal myogenesis progression via stage-specific functions. / Hsieh, C. L.; Liu, H.; Huang, Y.; Kang, L.; Chen, H. W.; Chen, Y. T.; Wee, Y. R.; Chen, S. J.; Tan, B. C M.

In: Cell Death and Differentiation, Vol. 21, No. 5, 2014, p. 707-719.

Research output: Contribution to journalArticle

Hsieh, CL, Liu, H, Huang, Y, Kang, L, Chen, HW, Chen, YT, Wee, YR, Chen, SJ & Tan, BCM 2014, 'ADAR1 deaminase contributes to scheduled skeletal myogenesis progression via stage-specific functions', Cell Death and Differentiation, vol. 21, no. 5, pp. 707-719. https://doi.org/10.1038/cdd.2013.197
Hsieh, C. L. ; Liu, H. ; Huang, Y. ; Kang, L. ; Chen, H. W. ; Chen, Y. T. ; Wee, Y. R. ; Chen, S. J. ; Tan, B. C M. / ADAR1 deaminase contributes to scheduled skeletal myogenesis progression via stage-specific functions. In: Cell Death and Differentiation. 2014 ; Vol. 21, No. 5. pp. 707-719.
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