Acute megakaryoblastic leukemia in childhood

Eric Sariban, Constance Oliver, Lawrence Corash, Jeffrey Cossman, Jackie Whang‐Peng, Elaine S. Jaffe, Harvey R. Gralnick, David G. Poplack

Research output: Contribution to journalArticle

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Abstract

Routine morphologic and cytochemical study of the leukemic cells of a 13‐month‐old child did not permit definitive determination of either a lymphoid or a myeloid cell origin. However, ultrastructural cytochemical analysis revealed platelet peroxidase (PPO) reactivity. The malignant cells expressed PPO in both unfixed and fixed preparations. PPO was observed in the perinuclear space and reticulum but not in the Golgi saccules. In a 1% glutaraldehyde, 1% formaldehyde solution known to inhibit PPO but not myeloperoxidase, no reaction product was observed. The demonstration of PPO activity in these undifferentiated cells established their megakaryoblastic lineage. Further studies including DNA flow cytometry confirmed that these cells were megakaryoblasts. This study demonstrates that megakaryoblastic malignancy may occur as an acute leukemia of childhood and emphasizes the value of ultrastructural cytochemistry in cases of acute leukemia which defy routine classification. Abnormalities of chromosome 21 were present in our patient as in four previous cases of megakaryoblastic leukemia in childhood. The nonrandomness of chromosome 21 involvement in this disease should therefore be considered.

Original languageEnglish
Pages (from-to)1423-1428
Number of pages6
JournalCancer
Volume54
Issue number7
DOIs
Publication statusPublished - Jan 1 1984
Externally publishedYes

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Leukemia, Megakaryoblastic, Acute
Peroxidase
Blood Platelets
Chromosomes, Human, Pair 21
Leukemia
Megakaryocyte Progenitor Cells
Saccule and Utricle
Histocytochemistry
Reticulum
Glutaral
Myeloid Cells
Formaldehyde
Flow Cytometry
DNA

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Sariban, E., Oliver, C., Corash, L., Cossman, J., Whang‐Peng, J., Jaffe, E. S., ... Poplack, D. G. (1984). Acute megakaryoblastic leukemia in childhood. Cancer, 54(7), 1423-1428. https://doi.org/10.1002/1097-0142(19841001)54:7<1423::AID-CNCR2820540732>3.0.CO;2-0

Acute megakaryoblastic leukemia in childhood. / Sariban, Eric; Oliver, Constance; Corash, Lawrence; Cossman, Jeffrey; Whang‐Peng, Jackie; Jaffe, Elaine S.; Gralnick, Harvey R.; Poplack, David G.

In: Cancer, Vol. 54, No. 7, 01.01.1984, p. 1423-1428.

Research output: Contribution to journalArticle

Sariban, E, Oliver, C, Corash, L, Cossman, J, Whang‐Peng, J, Jaffe, ES, Gralnick, HR & Poplack, DG 1984, 'Acute megakaryoblastic leukemia in childhood', Cancer, vol. 54, no. 7, pp. 1423-1428. https://doi.org/10.1002/1097-0142(19841001)54:7<1423::AID-CNCR2820540732>3.0.CO;2-0
Sariban, Eric ; Oliver, Constance ; Corash, Lawrence ; Cossman, Jeffrey ; Whang‐Peng, Jackie ; Jaffe, Elaine S. ; Gralnick, Harvey R. ; Poplack, David G. / Acute megakaryoblastic leukemia in childhood. In: Cancer. 1984 ; Vol. 54, No. 7. pp. 1423-1428.
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