Acute hypoxic preconditioning prevents palmitic acid-induced cardiomyocyte apoptosis via switching metabolic GLUT4-glucose pathway back to CD36-fatty acid dependent

Yeh Peng Chen, Wei Wen Kuo, Rathinasamy Baskaran, Cecilia Hsuan Day, Ray Jade Chen, Su Ying Wen, Tsung Jung Ho, Viswanadha Vijaya Padma, Chia Hua Kuo, Chih Yang Huang

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Abstract

Metabolic syndrome is a risk factor for the development of cardiovascular diseases. Myocardial cell damage leads to an imbalance of energy metabolism, and many studies have indicated that short-term hypoxia during myocardial cell injury has a protective effect. In our previous animal studies, we found that short-term hypoxia in the heart has a protective effect, but long-term hypoxia increases myocardial cell injury. Palmitic acid (PA)-treated H9c2 cardiomyoblasts and neonatal rat ventricle cardiomyocytes were used to simulate hyperlipidemia model, which suppress cluster of differentiation 36 (CD36) and activate glucose transporter type 4 (GLUT4). We exposed the cells to short- and long-term hypoxia and investigated the protective effects of hypoxic preconditioning on PA-induced lipotoxicity in H9c2 cardiomyoblasts and neonatal rat cardiomyocytes. Preconditioning with short-term hypoxia enhanced both CD36 and GLUT4 metabolism pathway protein levels. Expression levels of phospho-PI3K, phospho-Akt, phospho-AMPK, SIRT1, PGC1α, PPARα, CD36, and CPT1β induced by PA was reversed by short-term hypoxia in a time-dependent manner. PA-induced increased GLUT4 membrane protein level was reduced in the cells exposed to short-term hypoxia and si-PKCζ. Short-term hypoxia, resveratrol and si-PKCζ rescue H9c2 cells from apoptosis induced by PA and switch the metabolic pathway from GLUT4 dependent to CD36 dependent. We demonstrate short-term hypoxic preconditioning as a more efficient way as resveratrol in maintaining the energy metabolism of hearts during hyperlipidemia and can be used as a therapeutic strategy.

LanguageEnglish
Pages3363-3372
Number of pages10
JournalJournal of Cellular Biochemistry
Volume119
Issue number4
DOIs
Publication statusPublished - Apr 1 2018

Fingerprint

Glucose Transporter Type 4
Palmitic Acid
Cardiac Myocytes
Fatty Acids
Apoptosis
Glucose
Rats
Hyperlipidemias
AMP-Activated Protein Kinases
Energy Metabolism
Phosphatidylinositol 3-Kinases
Metabolism
Membrane Proteins
Animals
Cells
Switches
Hypoxia
Wounds and Injuries
Metabolic Networks and Pathways
Cardiovascular Diseases

Keywords

  • fatty acid metabolism
  • glucose metabolism
  • hypoxia
  • insulin signaling pathway
  • ischemia
  • palmitic acid

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Acute hypoxic preconditioning prevents palmitic acid-induced cardiomyocyte apoptosis via switching metabolic GLUT4-glucose pathway back to CD36-fatty acid dependent. / Chen, Yeh Peng; Kuo, Wei Wen; Baskaran, Rathinasamy; Day, Cecilia Hsuan; Chen, Ray Jade; Wen, Su Ying; Ho, Tsung Jung; Padma, Viswanadha Vijaya; Kuo, Chia Hua; Huang, Chih Yang.

In: Journal of Cellular Biochemistry, Vol. 119, No. 4, 01.04.2018, p. 3363-3372.

Research output: Contribution to journalArticle

Chen, Yeh Peng ; Kuo, Wei Wen ; Baskaran, Rathinasamy ; Day, Cecilia Hsuan ; Chen, Ray Jade ; Wen, Su Ying ; Ho, Tsung Jung ; Padma, Viswanadha Vijaya ; Kuo, Chia Hua ; Huang, Chih Yang. / Acute hypoxic preconditioning prevents palmitic acid-induced cardiomyocyte apoptosis via switching metabolic GLUT4-glucose pathway back to CD36-fatty acid dependent. In: Journal of Cellular Biochemistry. 2018 ; Vol. 119, No. 4. pp. 3363-3372.
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abstract = "Metabolic syndrome is a risk factor for the development of cardiovascular diseases. Myocardial cell damage leads to an imbalance of energy metabolism, and many studies have indicated that short-term hypoxia during myocardial cell injury has a protective effect. In our previous animal studies, we found that short-term hypoxia in the heart has a protective effect, but long-term hypoxia increases myocardial cell injury. Palmitic acid (PA)-treated H9c2 cardiomyoblasts and neonatal rat ventricle cardiomyocytes were used to simulate hyperlipidemia model, which suppress cluster of differentiation 36 (CD36) and activate glucose transporter type 4 (GLUT4). We exposed the cells to short- and long-term hypoxia and investigated the protective effects of hypoxic preconditioning on PA-induced lipotoxicity in H9c2 cardiomyoblasts and neonatal rat cardiomyocytes. Preconditioning with short-term hypoxia enhanced both CD36 and GLUT4 metabolism pathway protein levels. Expression levels of phospho-PI3K, phospho-Akt, phospho-AMPK, SIRT1, PGC1α, PPARα, CD36, and CPT1β induced by PA was reversed by short-term hypoxia in a time-dependent manner. PA-induced increased GLUT4 membrane protein level was reduced in the cells exposed to short-term hypoxia and si-PKCζ. Short-term hypoxia, resveratrol and si-PKCζ rescue H9c2 cells from apoptosis induced by PA and switch the metabolic pathway from GLUT4 dependent to CD36 dependent. We demonstrate short-term hypoxic preconditioning as a more efficient way as resveratrol in maintaining the energy metabolism of hearts during hyperlipidemia and can be used as a therapeutic strategy.",
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AU - Kuo, Wei Wen

AU - Baskaran, Rathinasamy

AU - Day, Cecilia Hsuan

AU - Chen, Ray Jade

AU - Wen, Su Ying

AU - Ho, Tsung Jung

AU - Padma, Viswanadha Vijaya

AU - Kuo, Chia Hua

AU - Huang, Chih Yang

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KW - fatty acid metabolism

KW - glucose metabolism

KW - hypoxia

KW - insulin signaling pathway

KW - ischemia

KW - palmitic acid

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