Acute anal stretch inhibits NMDA-dependent pelvic-urethra reflex potentiation via spinal GABAergic inhibition in anesthetized rats

Sung Lang Chen, Yu Hui Huang, Yu Lin Kao, Gin Den Chen, Chen Li Cheng, Hsien Yu Peng, Jiuan Miaw Liao, Pei Chen Huang, Shih Jei Tsai, Tzer Bin Lin

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The impact of acute anal stretch on the pelvic-urethra reflex potentiation was examined in urethane-anesthetized rats by recording the external urethra sphincter electromyogram activity evoked by the pelvic afferent stimulation. Test stimulation (1 stimulation/30 s) evoked a baseline reflex activity with a single action potential that was abolished by gallamine (5 mg/kg iv). On the other hand, the repetitive stimulation (1 stimulation/1 s) induced spinal reflex potentiation (SRP) that was attenuated by intrathecal 6-cyano-7- nitroquinoxaline-2,4-dione (a glutamatergic α-amino-3-hydroxy-5-methyl-4- isoxazoleproprionat receptor antagonist, 100 μM, 10 μl) and D-2-amino-5-phosphonovalerate [a glutamatergic N-methyl-D-aspartate (NMDA) antagonist, 100 μM, 10 μl]. Acute anal stretch using a mosquito clamp with a distance of 4 mm exhibited no effect, whereas distances of 8 mm attenuated and 12 mm abolished the repetitive stimulation-induced SRP. Intrathecal NMDA (100 μM, 10 μl) reversed the abolition on SRP caused by anal stretch. On the other hand, pretreated bicuculline [γ-aminobutyric acid (GABA) A receptor antagonist, 100 μM, 10 μl] but not hydroxysaclofen (GABAB receptor antagonist) counteracted the abolition on the repetitive stimulation-induced SRP caused by the anal stretch. All of the results suggested that anal stretch may be used as an adjunct to assist voiding dysfunction in patients with overactive urethra sphincter and that GABAergic neurotransmission is important in the neural mechanisms underlying external urethra sphincter activity inhibited by anal stretch.

Original languageEnglish
JournalAmerican Journal of Physiology - Renal Physiology
Volume295
Issue number4
DOIs
Publication statusPublished - Oct 2008
Externally publishedYes

Fingerprint

Urethra
N-Methylaspartate
Reflex
Gallamine Triethiodide
2-Amino-5-phosphonovalerate
Aminobutyrates
GABA-A Receptor Antagonists
Bicuculline
Urethane
Electromyography
Culicidae
Synaptic Transmission
Action Potentials
Inhibition (Psychology)

Keywords

  • Bicuculline
  • Glutamate
  • Hydroxysaclofen
  • Spinal cord
  • Voiding dysfunction

ASJC Scopus subject areas

  • Physiology
  • Urology

Cite this

Acute anal stretch inhibits NMDA-dependent pelvic-urethra reflex potentiation via spinal GABAergic inhibition in anesthetized rats. / Chen, Sung Lang; Huang, Yu Hui; Kao, Yu Lin; Chen, Gin Den; Cheng, Chen Li; Peng, Hsien Yu; Liao, Jiuan Miaw; Huang, Pei Chen; Tsai, Shih Jei; Lin, Tzer Bin.

In: American Journal of Physiology - Renal Physiology, Vol. 295, No. 4, 10.2008.

Research output: Contribution to journalArticle

Chen, Sung Lang ; Huang, Yu Hui ; Kao, Yu Lin ; Chen, Gin Den ; Cheng, Chen Li ; Peng, Hsien Yu ; Liao, Jiuan Miaw ; Huang, Pei Chen ; Tsai, Shih Jei ; Lin, Tzer Bin. / Acute anal stretch inhibits NMDA-dependent pelvic-urethra reflex potentiation via spinal GABAergic inhibition in anesthetized rats. In: American Journal of Physiology - Renal Physiology. 2008 ; Vol. 295, No. 4.
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abstract = "The impact of acute anal stretch on the pelvic-urethra reflex potentiation was examined in urethane-anesthetized rats by recording the external urethra sphincter electromyogram activity evoked by the pelvic afferent stimulation. Test stimulation (1 stimulation/30 s) evoked a baseline reflex activity with a single action potential that was abolished by gallamine (5 mg/kg iv). On the other hand, the repetitive stimulation (1 stimulation/1 s) induced spinal reflex potentiation (SRP) that was attenuated by intrathecal 6-cyano-7- nitroquinoxaline-2,4-dione (a glutamatergic α-amino-3-hydroxy-5-methyl-4- isoxazoleproprionat receptor antagonist, 100 μM, 10 μl) and D-2-amino-5-phosphonovalerate [a glutamatergic N-methyl-D-aspartate (NMDA) antagonist, 100 μM, 10 μl]. Acute anal stretch using a mosquito clamp with a distance of 4 mm exhibited no effect, whereas distances of 8 mm attenuated and 12 mm abolished the repetitive stimulation-induced SRP. Intrathecal NMDA (100 μM, 10 μl) reversed the abolition on SRP caused by anal stretch. On the other hand, pretreated bicuculline [γ-aminobutyric acid (GABA) A receptor antagonist, 100 μM, 10 μl] but not hydroxysaclofen (GABAB receptor antagonist) counteracted the abolition on the repetitive stimulation-induced SRP caused by the anal stretch. All of the results suggested that anal stretch may be used as an adjunct to assist voiding dysfunction in patients with overactive urethra sphincter and that GABAergic neurotransmission is important in the neural mechanisms underlying external urethra sphincter activity inhibited by anal stretch.",
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AU - Huang, Yu Hui

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AU - Chen, Gin Den

AU - Cheng, Chen Li

AU - Peng, Hsien Yu

AU - Liao, Jiuan Miaw

AU - Huang, Pei Chen

AU - Tsai, Shih Jei

AU - Lin, Tzer Bin

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AB - The impact of acute anal stretch on the pelvic-urethra reflex potentiation was examined in urethane-anesthetized rats by recording the external urethra sphincter electromyogram activity evoked by the pelvic afferent stimulation. Test stimulation (1 stimulation/30 s) evoked a baseline reflex activity with a single action potential that was abolished by gallamine (5 mg/kg iv). On the other hand, the repetitive stimulation (1 stimulation/1 s) induced spinal reflex potentiation (SRP) that was attenuated by intrathecal 6-cyano-7- nitroquinoxaline-2,4-dione (a glutamatergic α-amino-3-hydroxy-5-methyl-4- isoxazoleproprionat receptor antagonist, 100 μM, 10 μl) and D-2-amino-5-phosphonovalerate [a glutamatergic N-methyl-D-aspartate (NMDA) antagonist, 100 μM, 10 μl]. Acute anal stretch using a mosquito clamp with a distance of 4 mm exhibited no effect, whereas distances of 8 mm attenuated and 12 mm abolished the repetitive stimulation-induced SRP. Intrathecal NMDA (100 μM, 10 μl) reversed the abolition on SRP caused by anal stretch. On the other hand, pretreated bicuculline [γ-aminobutyric acid (GABA) A receptor antagonist, 100 μM, 10 μl] but not hydroxysaclofen (GABAB receptor antagonist) counteracted the abolition on the repetitive stimulation-induced SRP caused by the anal stretch. All of the results suggested that anal stretch may be used as an adjunct to assist voiding dysfunction in patients with overactive urethra sphincter and that GABAergic neurotransmission is important in the neural mechanisms underlying external urethra sphincter activity inhibited by anal stretch.

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