Activity staining on polyacrylamide gels of trypsin inhibitors from leaves of sweet potato (Ipomoea batatas L. Lam) varieties

Wen Chi Hou, Yaw Huei Lin

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The failure of activity staining of trypsin inhibitors in crude leaf extracts of sweet potato varieties including Tainong 27, Tainong 34, and Tainong 57: on a 15 % sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gel was prevented by dipping the gels in solutions containing 10-40 mM hydrogen peroxide, 10 mM Tris buffer (pH 7.9) for 30 min before staining.

Original languageEnglish
Pages (from-to)212-214
Number of pages3
JournalElectrophoresis
Volume19
Issue number2
DOIs
Publication statusPublished - Feb 1998
Externally publishedYes

Fingerprint

Ipomoea batatas
Trypsin Inhibitors
Gels
Staining and Labeling
Tromethamine
Electrophoresis
Complex Mixtures
Sodium Dodecyl Sulfate
Hydrogen Peroxide
Polyacrylamide Gel Electrophoresis
polyacrylamide gels

Keywords

  • Activity staining
  • Ipomoea batatas (L) Lam
  • Leaves
  • Sodium dodecyl sulfate -polyacrylamide gel electrophoresis
  • Trypsin inhibitors

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Activity staining on polyacrylamide gels of trypsin inhibitors from leaves of sweet potato (Ipomoea batatas L. Lam) varieties. / Hou, Wen Chi; Lin, Yaw Huei.

In: Electrophoresis, Vol. 19, No. 2, 02.1998, p. 212-214.

Research output: Contribution to journalArticle

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