Activity staining of pectinesterase on-polyacrylamide gels after acidic or sodium dodecyl sulfate electrophoresis

Wen Chi Hou, Yaw Huei Lin

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17 Citations (Scopus)

Abstract

Pectinesterase (PE), from commercial orange peels or ammonium sulfate fractionation (50-80% saturation) of pea pods, was detected on polyacrylamide gels after native acidic polyacrylamide gel electrophoresis (PAGE) or sodium dodecyl sulfate (SDS)-PAGE by using the synthetic substrate β-naphthyl acetate (β-NA). The release of β-naphthol (at 322 nm) from β-NA was proportional to PE activity. The PE activity bands on polyacrylamide gels after native acidic PAGE or SDS-PAGE were stained with a combination of tetrazotized o-dianisidine and β-NA. This fast and sensitive method can be used for enzyme purification and characterization.

Original languageEnglish
Pages (from-to)692-694
Number of pages3
JournalElectrophoresis
Volume19
Issue number5
DOIs
Publication statusPublished - May 1998
Externally publishedYes

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Keywords

  • Activity staining
  • Native acidic polyacrylamide gel electrophoresis
  • Pectinesterase

ASJC Scopus subject areas

  • Clinical Biochemistry

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