Activity staining of pectinesterase on-polyacrylamide gels after acidic or sodium dodecyl sulfate electrophoresis

Wen Chi Hou, Yaw Huei Lin

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Pectinesterase (PE), from commercial orange peels or ammonium sulfate fractionation (50-80% saturation) of pea pods, was detected on polyacrylamide gels after native acidic polyacrylamide gel electrophoresis (PAGE) or sodium dodecyl sulfate (SDS)-PAGE by using the synthetic substrate β-naphthyl acetate (β-NA). The release of β-naphthol (at 322 nm) from β-NA was proportional to PE activity. The PE activity bands on polyacrylamide gels after native acidic PAGE or SDS-PAGE were stained with a combination of tetrazotized o-dianisidine and β-NA. This fast and sensitive method can be used for enzyme purification and characterization.

Original languageEnglish
Pages (from-to)692-694
Number of pages3
JournalElectrophoresis
Volume19
Issue number5
DOIs
Publication statusPublished - May 1998
Externally publishedYes

Fingerprint

Electrophoresis
Sodium Dodecyl Sulfate
Native Polyacrylamide Gel Electrophoresis
Staining and Labeling
Polyacrylamide Gel Electrophoresis
Dianisidine
Naphthols
Peas
Ammonium Sulfate
Enzymes
Fractionation
Purification
pectinesterase
polyacrylamide gels
Substrates

Keywords

  • Activity staining
  • Native acidic polyacrylamide gel electrophoresis
  • Pectinesterase

ASJC Scopus subject areas

  • Clinical Biochemistry

Cite this

Activity staining of pectinesterase on-polyacrylamide gels after acidic or sodium dodecyl sulfate electrophoresis. / Hou, Wen Chi; Lin, Yaw Huei.

In: Electrophoresis, Vol. 19, No. 5, 05.1998, p. 692-694.

Research output: Contribution to journalArticle

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