Activities of superoxide dismutase and glutathione peroxidase in leaves of different cultivars of Liriope spicata L. on 10% SDS-PAGE gels

Wen C. Hou, Yeh L. Lu, Sin Y. Liu, Yaw Huei Lin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in leaf crude extracts of different cultivars (small leaf, SL; big leaf, BL; thin leaf, TL) of Liriope spicata L. were detected on 10% SDS-PAGE gels. All cultivars contained different Cu/Zn SOD isozymes with molecular masses between 30 and 50 kDa, which were identified by inhibitor tests. They also contained different GPx isozymes with molecular masses between 50 and 64 kDa. It was found that SOD isozymes were stable during 40 to 65°C treatment; however, no SOD activity could be detected at either 70 or 80°C for 5 min. In contrast, all GPx isozymes were stable under 80°C for 5 min, and GPx in SL and TL cultivars could resist 80°C treatment for 30 min.

Original languageEnglish
Pages (from-to)37-41
Number of pages5
JournalBotanical Bulletin of Academia Sinica
Volume44
Issue number1
Publication statusPublished - Jan 2003

Fingerprint

Liriope spicata
glutathione peroxidase
isozymes
superoxide dismutase
gels
cultivars
leaves
molecular weight
extracts
testing

Keywords

  • Glutathione peroxidase
  • Liriope spicata L.
  • Superoxide dismutase

ASJC Scopus subject areas

  • Plant Science

Cite this

Activities of superoxide dismutase and glutathione peroxidase in leaves of different cultivars of Liriope spicata L. on 10% SDS-PAGE gels. / Hou, Wen C.; Lu, Yeh L.; Liu, Sin Y.; Lin, Yaw Huei.

In: Botanical Bulletin of Academia Sinica, Vol. 44, No. 1, 01.2003, p. 37-41.

Research output: Contribution to journalArticle

@article{ee8a1e18ee9a4e1e9dc2c2af567b42af,
title = "Activities of superoxide dismutase and glutathione peroxidase in leaves of different cultivars of Liriope spicata L. on 10{\%} SDS-PAGE gels",
abstract = "Activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in leaf crude extracts of different cultivars (small leaf, SL; big leaf, BL; thin leaf, TL) of Liriope spicata L. were detected on 10{\%} SDS-PAGE gels. All cultivars contained different Cu/Zn SOD isozymes with molecular masses between 30 and 50 kDa, which were identified by inhibitor tests. They also contained different GPx isozymes with molecular masses between 50 and 64 kDa. It was found that SOD isozymes were stable during 40 to 65°C treatment; however, no SOD activity could be detected at either 70 or 80°C for 5 min. In contrast, all GPx isozymes were stable under 80°C for 5 min, and GPx in SL and TL cultivars could resist 80°C treatment for 30 min.",
keywords = "Glutathione peroxidase, Liriope spicata L., Superoxide dismutase",
author = "Hou, {Wen C.} and Lu, {Yeh L.} and Liu, {Sin Y.} and Lin, {Yaw Huei}",
year = "2003",
month = "1",
language = "English",
volume = "44",
pages = "37--41",
journal = "Botanical Studies",
issn = "1817-406X",
publisher = "Academia Sinica",
number = "1",

}

TY - JOUR

T1 - Activities of superoxide dismutase and glutathione peroxidase in leaves of different cultivars of Liriope spicata L. on 10% SDS-PAGE gels

AU - Hou, Wen C.

AU - Lu, Yeh L.

AU - Liu, Sin Y.

AU - Lin, Yaw Huei

PY - 2003/1

Y1 - 2003/1

N2 - Activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in leaf crude extracts of different cultivars (small leaf, SL; big leaf, BL; thin leaf, TL) of Liriope spicata L. were detected on 10% SDS-PAGE gels. All cultivars contained different Cu/Zn SOD isozymes with molecular masses between 30 and 50 kDa, which were identified by inhibitor tests. They also contained different GPx isozymes with molecular masses between 50 and 64 kDa. It was found that SOD isozymes were stable during 40 to 65°C treatment; however, no SOD activity could be detected at either 70 or 80°C for 5 min. In contrast, all GPx isozymes were stable under 80°C for 5 min, and GPx in SL and TL cultivars could resist 80°C treatment for 30 min.

AB - Activities of superoxide dismutase (SOD) and glutathione peroxidase (GPx) in leaf crude extracts of different cultivars (small leaf, SL; big leaf, BL; thin leaf, TL) of Liriope spicata L. were detected on 10% SDS-PAGE gels. All cultivars contained different Cu/Zn SOD isozymes with molecular masses between 30 and 50 kDa, which were identified by inhibitor tests. They also contained different GPx isozymes with molecular masses between 50 and 64 kDa. It was found that SOD isozymes were stable during 40 to 65°C treatment; however, no SOD activity could be detected at either 70 or 80°C for 5 min. In contrast, all GPx isozymes were stable under 80°C for 5 min, and GPx in SL and TL cultivars could resist 80°C treatment for 30 min.

KW - Glutathione peroxidase

KW - Liriope spicata L.

KW - Superoxide dismutase

UR - http://www.scopus.com/inward/record.url?scp=0037284273&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037284273&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0037284273

VL - 44

SP - 37

EP - 41

JO - Botanical Studies

JF - Botanical Studies

SN - 1817-406X

IS - 1

ER -