Activation of the iberiotoxin-sensitive BKCa channels by salvianolic acid B of the porcine coronary artery smooth muscle cells

Francis Fu Yuen Lam, Sai Wang Seto, Yiu Wa Kwan, John Hok Keung Yeung, Paul Chan

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

In this study, we examined the effects of Salvia miltiorrhiza (Danshen) crude extract, some of its lipid-soluble components (tanshinone I, tanshinone IIA, cryptotanshinone, dihydroisotanshinone I) and the water-soluble compounds (danshensu and salvianolic acid B) on the K+ channels such as the iberiotoxin-sensitive Ca2+-activated K+ (BKCa) channels and the glibenclamide-sensitive ATP-dependent K+ (IKATP) channels of the porcine left anterior descending coronary artery smooth muscle cells. Cumulative application of salvianolic acid B (30-300 μM) caused a l-NNA (100 μM)-insensitive, potentiation of the outward BKCa current amplitude with no apparent effect on the IKATP channels opening. Salvianolic acid B (300 μM) caused an ODQ (10 μM, a guanylate cyclase inhibitor)-sensitive enhancement of the outward BKCa current amplitude. In contrast, none of the other isolated chemical constituents of S. miltiorrhiza modified the openings of the two types of K+ channels studied. In conclusion, our results suggest that salvianolic acid B, a major hydrophilic constituent found in Radix S. miltiorrhiza, activated the opening of the BKCa channels of the porcine coronary artery smooth muscle cells through the activation of guanylate cyclase without the involvement of the nitric oxide synthase activation.

Original languageEnglish
Pages (from-to)28-35
Number of pages8
JournalEuropean Journal of Pharmacology
Volume546
Issue number1-3
DOIs
Publication statusPublished - Sep 28 2006

Fingerprint

Salvia miltiorrhiza
Smooth Muscle Myocytes
Coronary Vessels
Swine
Guanylate Cyclase
Calcium-Activated Potassium Channels
Glyburide
Complex Mixtures
Nitric Oxide Synthase
Adenosine Triphosphate
Lipids
iberiotoxin
salvianolic acid B
Water
tanshinone

Keywords

  • (Salvia miltiorrhiza (Danshen))
  • BK channels
  • Porcine coronary artery
  • Salvianolic acid B

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Activation of the iberiotoxin-sensitive BKCa channels by salvianolic acid B of the porcine coronary artery smooth muscle cells. / Lam, Francis Fu Yuen; Seto, Sai Wang; Kwan, Yiu Wa; Yeung, John Hok Keung; Chan, Paul.

In: European Journal of Pharmacology, Vol. 546, No. 1-3, 28.09.2006, p. 28-35.

Research output: Contribution to journalArticle

Lam, Francis Fu Yuen ; Seto, Sai Wang ; Kwan, Yiu Wa ; Yeung, John Hok Keung ; Chan, Paul. / Activation of the iberiotoxin-sensitive BKCa channels by salvianolic acid B of the porcine coronary artery smooth muscle cells. In: European Journal of Pharmacology. 2006 ; Vol. 546, No. 1-3. pp. 28-35.
@article{f2be9ff63ea14dc99e5d9b6bb13ca948,
title = "Activation of the iberiotoxin-sensitive BKCa channels by salvianolic acid B of the porcine coronary artery smooth muscle cells",
abstract = "In this study, we examined the effects of Salvia miltiorrhiza (Danshen) crude extract, some of its lipid-soluble components (tanshinone I, tanshinone IIA, cryptotanshinone, dihydroisotanshinone I) and the water-soluble compounds (danshensu and salvianolic acid B) on the K+ channels such as the iberiotoxin-sensitive Ca2+-activated K+ (BKCa) channels and the glibenclamide-sensitive ATP-dependent K+ (IKATP) channels of the porcine left anterior descending coronary artery smooth muscle cells. Cumulative application of salvianolic acid B (30-300 μM) caused a l-NNA (100 μM)-insensitive, potentiation of the outward BKCa current amplitude with no apparent effect on the IKATP channels opening. Salvianolic acid B (300 μM) caused an ODQ (10 μM, a guanylate cyclase inhibitor)-sensitive enhancement of the outward BKCa current amplitude. In contrast, none of the other isolated chemical constituents of S. miltiorrhiza modified the openings of the two types of K+ channels studied. In conclusion, our results suggest that salvianolic acid B, a major hydrophilic constituent found in Radix S. miltiorrhiza, activated the opening of the BKCa channels of the porcine coronary artery smooth muscle cells through the activation of guanylate cyclase without the involvement of the nitric oxide synthase activation.",
keywords = "(Salvia miltiorrhiza (Danshen)), BK channels, Porcine coronary artery, Salvianolic acid B",
author = "Lam, {Francis Fu Yuen} and Seto, {Sai Wang} and Kwan, {Yiu Wa} and Yeung, {John Hok Keung} and Paul Chan",
year = "2006",
month = "9",
day = "28",
doi = "10.1016/j.ejphar.2006.07.038",
language = "English",
volume = "546",
pages = "28--35",
journal = "European Journal of Pharmacology",
issn = "0014-2999",
publisher = "Elsevier",
number = "1-3",

}

TY - JOUR

T1 - Activation of the iberiotoxin-sensitive BKCa channels by salvianolic acid B of the porcine coronary artery smooth muscle cells

AU - Lam, Francis Fu Yuen

AU - Seto, Sai Wang

AU - Kwan, Yiu Wa

AU - Yeung, John Hok Keung

AU - Chan, Paul

PY - 2006/9/28

Y1 - 2006/9/28

N2 - In this study, we examined the effects of Salvia miltiorrhiza (Danshen) crude extract, some of its lipid-soluble components (tanshinone I, tanshinone IIA, cryptotanshinone, dihydroisotanshinone I) and the water-soluble compounds (danshensu and salvianolic acid B) on the K+ channels such as the iberiotoxin-sensitive Ca2+-activated K+ (BKCa) channels and the glibenclamide-sensitive ATP-dependent K+ (IKATP) channels of the porcine left anterior descending coronary artery smooth muscle cells. Cumulative application of salvianolic acid B (30-300 μM) caused a l-NNA (100 μM)-insensitive, potentiation of the outward BKCa current amplitude with no apparent effect on the IKATP channels opening. Salvianolic acid B (300 μM) caused an ODQ (10 μM, a guanylate cyclase inhibitor)-sensitive enhancement of the outward BKCa current amplitude. In contrast, none of the other isolated chemical constituents of S. miltiorrhiza modified the openings of the two types of K+ channels studied. In conclusion, our results suggest that salvianolic acid B, a major hydrophilic constituent found in Radix S. miltiorrhiza, activated the opening of the BKCa channels of the porcine coronary artery smooth muscle cells through the activation of guanylate cyclase without the involvement of the nitric oxide synthase activation.

AB - In this study, we examined the effects of Salvia miltiorrhiza (Danshen) crude extract, some of its lipid-soluble components (tanshinone I, tanshinone IIA, cryptotanshinone, dihydroisotanshinone I) and the water-soluble compounds (danshensu and salvianolic acid B) on the K+ channels such as the iberiotoxin-sensitive Ca2+-activated K+ (BKCa) channels and the glibenclamide-sensitive ATP-dependent K+ (IKATP) channels of the porcine left anterior descending coronary artery smooth muscle cells. Cumulative application of salvianolic acid B (30-300 μM) caused a l-NNA (100 μM)-insensitive, potentiation of the outward BKCa current amplitude with no apparent effect on the IKATP channels opening. Salvianolic acid B (300 μM) caused an ODQ (10 μM, a guanylate cyclase inhibitor)-sensitive enhancement of the outward BKCa current amplitude. In contrast, none of the other isolated chemical constituents of S. miltiorrhiza modified the openings of the two types of K+ channels studied. In conclusion, our results suggest that salvianolic acid B, a major hydrophilic constituent found in Radix S. miltiorrhiza, activated the opening of the BKCa channels of the porcine coronary artery smooth muscle cells through the activation of guanylate cyclase without the involvement of the nitric oxide synthase activation.

KW - (Salvia miltiorrhiza (Danshen))

KW - BK channels

KW - Porcine coronary artery

KW - Salvianolic acid B

UR - http://www.scopus.com/inward/record.url?scp=33748291559&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33748291559&partnerID=8YFLogxK

U2 - 10.1016/j.ejphar.2006.07.038

DO - 10.1016/j.ejphar.2006.07.038

M3 - Article

C2 - 16928370

AN - SCOPUS:33748291559

VL - 546

SP - 28

EP - 35

JO - European Journal of Pharmacology

JF - European Journal of Pharmacology

SN - 0014-2999

IS - 1-3

ER -