Abstract
In this study, we examined the effects of Salvia miltiorrhiza (Danshen) crude extract, some of its lipid-soluble components (tanshinone I, tanshinone IIA, cryptotanshinone, dihydroisotanshinone I) and the water-soluble compounds (danshensu and salvianolic acid B) on the K+ channels such as the iberiotoxin-sensitive Ca2+-activated K+ (BKCa) channels and the glibenclamide-sensitive ATP-dependent K+ (IKATP) channels of the porcine left anterior descending coronary artery smooth muscle cells. Cumulative application of salvianolic acid B (30-300 μM) caused a l-NNA (100 μM)-insensitive, potentiation of the outward BKCa current amplitude with no apparent effect on the IKATP channels opening. Salvianolic acid B (300 μM) caused an ODQ (10 μM, a guanylate cyclase inhibitor)-sensitive enhancement of the outward BKCa current amplitude. In contrast, none of the other isolated chemical constituents of S. miltiorrhiza modified the openings of the two types of K+ channels studied. In conclusion, our results suggest that salvianolic acid B, a major hydrophilic constituent found in Radix S. miltiorrhiza, activated the opening of the BKCa channels of the porcine coronary artery smooth muscle cells through the activation of guanylate cyclase without the involvement of the nitric oxide synthase activation.
| Original language | English |
|---|---|
| Pages (from-to) | 28-35 |
| Number of pages | 8 |
| Journal | European Journal of Pharmacology |
| Volume | 546 |
| Issue number | 1-3 |
| DOIs | |
| Publication status | Published - Sep 28 2006 |
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Keywords
- (Salvia miltiorrhiza (Danshen))
- BK channels
- Porcine coronary artery
- Salvianolic acid B
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience
- Pharmacology
Cite this
Activation of the iberiotoxin-sensitive BKCa channels by salvianolic acid B of the porcine coronary artery smooth muscle cells. / Lam, Francis Fu Yuen; Seto, Sai Wang; Kwan, Yiu Wa; Yeung, John Hok Keung; Chan, Paul.
In: European Journal of Pharmacology, Vol. 546, No. 1-3, 28.09.2006, p. 28-35.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Activation of the iberiotoxin-sensitive BKCa channels by salvianolic acid B of the porcine coronary artery smooth muscle cells
AU - Lam, Francis Fu Yuen
AU - Seto, Sai Wang
AU - Kwan, Yiu Wa
AU - Yeung, John Hok Keung
AU - Chan, Paul
PY - 2006/9/28
Y1 - 2006/9/28
N2 - In this study, we examined the effects of Salvia miltiorrhiza (Danshen) crude extract, some of its lipid-soluble components (tanshinone I, tanshinone IIA, cryptotanshinone, dihydroisotanshinone I) and the water-soluble compounds (danshensu and salvianolic acid B) on the K+ channels such as the iberiotoxin-sensitive Ca2+-activated K+ (BKCa) channels and the glibenclamide-sensitive ATP-dependent K+ (IKATP) channels of the porcine left anterior descending coronary artery smooth muscle cells. Cumulative application of salvianolic acid B (30-300 μM) caused a l-NNA (100 μM)-insensitive, potentiation of the outward BKCa current amplitude with no apparent effect on the IKATP channels opening. Salvianolic acid B (300 μM) caused an ODQ (10 μM, a guanylate cyclase inhibitor)-sensitive enhancement of the outward BKCa current amplitude. In contrast, none of the other isolated chemical constituents of S. miltiorrhiza modified the openings of the two types of K+ channels studied. In conclusion, our results suggest that salvianolic acid B, a major hydrophilic constituent found in Radix S. miltiorrhiza, activated the opening of the BKCa channels of the porcine coronary artery smooth muscle cells through the activation of guanylate cyclase without the involvement of the nitric oxide synthase activation.
AB - In this study, we examined the effects of Salvia miltiorrhiza (Danshen) crude extract, some of its lipid-soluble components (tanshinone I, tanshinone IIA, cryptotanshinone, dihydroisotanshinone I) and the water-soluble compounds (danshensu and salvianolic acid B) on the K+ channels such as the iberiotoxin-sensitive Ca2+-activated K+ (BKCa) channels and the glibenclamide-sensitive ATP-dependent K+ (IKATP) channels of the porcine left anterior descending coronary artery smooth muscle cells. Cumulative application of salvianolic acid B (30-300 μM) caused a l-NNA (100 μM)-insensitive, potentiation of the outward BKCa current amplitude with no apparent effect on the IKATP channels opening. Salvianolic acid B (300 μM) caused an ODQ (10 μM, a guanylate cyclase inhibitor)-sensitive enhancement of the outward BKCa current amplitude. In contrast, none of the other isolated chemical constituents of S. miltiorrhiza modified the openings of the two types of K+ channels studied. In conclusion, our results suggest that salvianolic acid B, a major hydrophilic constituent found in Radix S. miltiorrhiza, activated the opening of the BKCa channels of the porcine coronary artery smooth muscle cells through the activation of guanylate cyclase without the involvement of the nitric oxide synthase activation.
KW - (Salvia miltiorrhiza (Danshen))
KW - BK channels
KW - Porcine coronary artery
KW - Salvianolic acid B
UR - http://www.scopus.com/inward/record.url?scp=33748291559&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748291559&partnerID=8YFLogxK
U2 - 10.1016/j.ejphar.2006.07.038
DO - 10.1016/j.ejphar.2006.07.038
M3 - Article
C2 - 16928370
AN - SCOPUS:33748291559
VL - 546
SP - 28
EP - 35
JO - European Journal of Pharmacology
JF - European Journal of Pharmacology
SN - 0014-2999
IS - 1-3
ER -