Activation of the cellular oncogene c-erbB by ltr insertion

Molecular basis for induction of erythroblastosis by avian leukosis virus

Yuen Kai T Fung, Wynne G. Lewis, Lyman B. Crittenden, Hsing Jien Kung

Research output: Contribution to journalArticle

133 Citations (Scopus)

Abstract

Avian leukosis virus (ALV), a slowly oncogenic retrovirus, induces in chickens a variety of neoplasms, including lymphoid leukosis and erythroblastosis. In lymphoid leukosis, a cellular oncogene, c-myc, is activated by the insertion of ALV LTR. We provide evidence that ALV utilizes a similar mechanism in erythroblastosis induction by activating a different cellular oncogene, c-erbB. We report the isolation, from leukemic erythroblast DNA, of a clone that represents the viral-cell junction fragment and carried the ALV LTR and part of the c-erbB locus. Restriction and sequence analyses reveal that the LTR is located upstream from the erbB coding region and is oriented in the same transcriptional direction; such a structure would be compatible with the promoter-insertion type of activation. Our findings provide a molecular explanation for the multipotency of slowly oncogenic retroviruses.

Original languageEnglish
Pages (from-to)357-368
Number of pages12
JournalCell
Volume33
Issue number2
DOIs
Publication statusPublished - Jan 1 1983
Externally publishedYes

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Avian Leukosis Virus
Viruses
Oncogenes
Chemical activation
Retroviridae
Erythroblasts
myc Genes
Intercellular Junctions
Sequence Analysis
Chickens
Clone Cells
DNA
Neoplasms

ASJC Scopus subject areas

  • Cell Biology
  • Molecular Biology
  • Medicine(all)

Cite this

Activation of the cellular oncogene c-erbB by ltr insertion : Molecular basis for induction of erythroblastosis by avian leukosis virus. / Fung, Yuen Kai T; Lewis, Wynne G.; Crittenden, Lyman B.; Kung, Hsing Jien.

In: Cell, Vol. 33, No. 2, 01.01.1983, p. 357-368.

Research output: Contribution to journalArticle

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