Abstract
Rta is a transcription factor encoded by BRLF1 of the Epstein-Barr virus (EBV). This factor is expressed during the immediate-early stage of the lytic cycle to activate the genes required for EBV lytic development. Although transcription activation by Rta is frequently associated with the binding of Rta to the Rta-response element (RRE) in promoters, Rta sometimes activates promoters without an RRE. Here we show that Rta interacts with an Sp1-interacting protein, MBD1-containing chromatin-associated factor 1 (MCAF1). This interaction is critical to the formation of an Sp1-MCAF1-Rta complex at Sp1 sites. Therefore, following lytic induction and the expression of Rta, Rta increases Sp1 -mediated transcription. The genes that are thus activated include p16, p21, SNRPN and BRLF1. However, the binding of Rta to RRE prevents the interaction between Rta and MCAF1; therefore, transcription activation by RRE depends only on Rta, and not on MCAF1 or Sp1. Furthermore, this study finds that MCAF1 promotes the expression of Rta and Zta from EBV, indicating that MCAF1 participates EBV lytic activation. Our study documents the critical role of Rta in regulating the transcription of the genes that are mediated by Sp1.
| Original language | English |
|---|---|
| Pages (from-to) | 6528-6539 |
| Number of pages | 12 |
| Journal | Nucleic Acids Research |
| Volume | 33 |
| Issue number | 20 |
| DOIs | |
| Publication status | Published - 2005 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Genetics
Cite this
Activation of Sp1-mediated transcription by Rta of Epstein-Barr virus via an interaction with MCAF1. / Chang, Li Kwan; Chung, Jian Ying; Hong, Yi Ren; Ichimura, Takaya; Nakao, Mitsuyoshi; Liu, Shih Tung.
In: Nucleic Acids Research, Vol. 33, No. 20, 2005, p. 6528-6539.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Activation of Sp1-mediated transcription by Rta of Epstein-Barr virus via an interaction with MCAF1
AU - Chang, Li Kwan
AU - Chung, Jian Ying
AU - Hong, Yi Ren
AU - Ichimura, Takaya
AU - Nakao, Mitsuyoshi
AU - Liu, Shih Tung
PY - 2005
Y1 - 2005
N2 - Rta is a transcription factor encoded by BRLF1 of the Epstein-Barr virus (EBV). This factor is expressed during the immediate-early stage of the lytic cycle to activate the genes required for EBV lytic development. Although transcription activation by Rta is frequently associated with the binding of Rta to the Rta-response element (RRE) in promoters, Rta sometimes activates promoters without an RRE. Here we show that Rta interacts with an Sp1-interacting protein, MBD1-containing chromatin-associated factor 1 (MCAF1). This interaction is critical to the formation of an Sp1-MCAF1-Rta complex at Sp1 sites. Therefore, following lytic induction and the expression of Rta, Rta increases Sp1 -mediated transcription. The genes that are thus activated include p16, p21, SNRPN and BRLF1. However, the binding of Rta to RRE prevents the interaction between Rta and MCAF1; therefore, transcription activation by RRE depends only on Rta, and not on MCAF1 or Sp1. Furthermore, this study finds that MCAF1 promotes the expression of Rta and Zta from EBV, indicating that MCAF1 participates EBV lytic activation. Our study documents the critical role of Rta in regulating the transcription of the genes that are mediated by Sp1.
AB - Rta is a transcription factor encoded by BRLF1 of the Epstein-Barr virus (EBV). This factor is expressed during the immediate-early stage of the lytic cycle to activate the genes required for EBV lytic development. Although transcription activation by Rta is frequently associated with the binding of Rta to the Rta-response element (RRE) in promoters, Rta sometimes activates promoters without an RRE. Here we show that Rta interacts with an Sp1-interacting protein, MBD1-containing chromatin-associated factor 1 (MCAF1). This interaction is critical to the formation of an Sp1-MCAF1-Rta complex at Sp1 sites. Therefore, following lytic induction and the expression of Rta, Rta increases Sp1 -mediated transcription. The genes that are thus activated include p16, p21, SNRPN and BRLF1. However, the binding of Rta to RRE prevents the interaction between Rta and MCAF1; therefore, transcription activation by RRE depends only on Rta, and not on MCAF1 or Sp1. Furthermore, this study finds that MCAF1 promotes the expression of Rta and Zta from EBV, indicating that MCAF1 participates EBV lytic activation. Our study documents the critical role of Rta in regulating the transcription of the genes that are mediated by Sp1.
UR - http://www.scopus.com/inward/record.url?scp=29144521749&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=29144521749&partnerID=8YFLogxK
U2 - 10.1093/nar/gki956
DO - 10.1093/nar/gki956
M3 - Article
C2 - 16314315
AN - SCOPUS:29144521749
VL - 33
SP - 6528
EP - 6539
JO - Nucleic Acids Research
JF - Nucleic Acids Research
SN - 0305-1048
IS - 20
ER -