Activation of opioid μ-receptor by sinomenine in cell and mice

Mao Hsien Wang, Cheng Kuei Chang, Jun Hwa Cheng, Hung Tsung Wu, Ying Xiao Li, Juei Tang Cheng

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Sinomenine, one of the alkaloids extracted from roots or stems of Sinomenium acutum, is documented to show antinociceptive action but the action mechanism is still unclear. The present study was aimed to investigate the effect of sinomenine on opioid μ-receptor (OMR). In Chinese Hamster Ovary (CHO) cell transfected with OMR, the binding of [3H]naloxone was displaced by sinomenine in a concentration-dependent manner. This compound also raised the phosphorylation of OMR in these cells. In a tail-flick test, sinomenine produced dose-dependent antinociception in mice, which was dose-dependently inhibited by pretreatment of naloxonazine, a selective OMR antagonist. Long-term pretreatment with sinomenine may delay the analgesic tolerance of morphine. The obtained results suggest that sinomenine has an ability to activate OMR, implicating the potential of sinomenine to be applied in clinic.

Original languageEnglish
Pages (from-to)209-212
Number of pages4
JournalNeuroscience Letters
Volume443
Issue number3
DOIs
Publication statusPublished - Oct 10 2008
Externally publishedYes

Fingerprint

Opioid Receptors
Sinomenium
Naloxone
Cricetulus
sinomenine
Alkaloids
Morphine
Analgesics
Tail
Ovary
Phosphorylation

Keywords

  • Antinociception
  • Mouse
  • Opioid μ-receptor
  • Radioligand binding
  • Receptor phosphorylation
  • Sinomenine
  • Transfected cell

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Activation of opioid μ-receptor by sinomenine in cell and mice. / Wang, Mao Hsien; Chang, Cheng Kuei; Cheng, Jun Hwa; Wu, Hung Tsung; Li, Ying Xiao; Cheng, Juei Tang.

In: Neuroscience Letters, Vol. 443, No. 3, 10.10.2008, p. 209-212.

Research output: Contribution to journalArticle

Wang, Mao Hsien ; Chang, Cheng Kuei ; Cheng, Jun Hwa ; Wu, Hung Tsung ; Li, Ying Xiao ; Cheng, Juei Tang. / Activation of opioid μ-receptor by sinomenine in cell and mice. In: Neuroscience Letters. 2008 ; Vol. 443, No. 3. pp. 209-212.
@article{0b65c4f49e13465f86075a631bcb102a,
title = "Activation of opioid μ-receptor by sinomenine in cell and mice",
abstract = "Sinomenine, one of the alkaloids extracted from roots or stems of Sinomenium acutum, is documented to show antinociceptive action but the action mechanism is still unclear. The present study was aimed to investigate the effect of sinomenine on opioid μ-receptor (OMR). In Chinese Hamster Ovary (CHO) cell transfected with OMR, the binding of [3H]naloxone was displaced by sinomenine in a concentration-dependent manner. This compound also raised the phosphorylation of OMR in these cells. In a tail-flick test, sinomenine produced dose-dependent antinociception in mice, which was dose-dependently inhibited by pretreatment of naloxonazine, a selective OMR antagonist. Long-term pretreatment with sinomenine may delay the analgesic tolerance of morphine. The obtained results suggest that sinomenine has an ability to activate OMR, implicating the potential of sinomenine to be applied in clinic.",
keywords = "Antinociception, Mouse, Opioid μ-receptor, Radioligand binding, Receptor phosphorylation, Sinomenine, Transfected cell",
author = "Wang, {Mao Hsien} and Chang, {Cheng Kuei} and Cheng, {Jun Hwa} and Wu, {Hung Tsung} and Li, {Ying Xiao} and Cheng, {Juei Tang}",
year = "2008",
month = "10",
day = "10",
doi = "10.1016/j.neulet.2008.07.088",
language = "English",
volume = "443",
pages = "209--212",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - Activation of opioid μ-receptor by sinomenine in cell and mice

AU - Wang, Mao Hsien

AU - Chang, Cheng Kuei

AU - Cheng, Jun Hwa

AU - Wu, Hung Tsung

AU - Li, Ying Xiao

AU - Cheng, Juei Tang

PY - 2008/10/10

Y1 - 2008/10/10

N2 - Sinomenine, one of the alkaloids extracted from roots or stems of Sinomenium acutum, is documented to show antinociceptive action but the action mechanism is still unclear. The present study was aimed to investigate the effect of sinomenine on opioid μ-receptor (OMR). In Chinese Hamster Ovary (CHO) cell transfected with OMR, the binding of [3H]naloxone was displaced by sinomenine in a concentration-dependent manner. This compound also raised the phosphorylation of OMR in these cells. In a tail-flick test, sinomenine produced dose-dependent antinociception in mice, which was dose-dependently inhibited by pretreatment of naloxonazine, a selective OMR antagonist. Long-term pretreatment with sinomenine may delay the analgesic tolerance of morphine. The obtained results suggest that sinomenine has an ability to activate OMR, implicating the potential of sinomenine to be applied in clinic.

AB - Sinomenine, one of the alkaloids extracted from roots or stems of Sinomenium acutum, is documented to show antinociceptive action but the action mechanism is still unclear. The present study was aimed to investigate the effect of sinomenine on opioid μ-receptor (OMR). In Chinese Hamster Ovary (CHO) cell transfected with OMR, the binding of [3H]naloxone was displaced by sinomenine in a concentration-dependent manner. This compound also raised the phosphorylation of OMR in these cells. In a tail-flick test, sinomenine produced dose-dependent antinociception in mice, which was dose-dependently inhibited by pretreatment of naloxonazine, a selective OMR antagonist. Long-term pretreatment with sinomenine may delay the analgesic tolerance of morphine. The obtained results suggest that sinomenine has an ability to activate OMR, implicating the potential of sinomenine to be applied in clinic.

KW - Antinociception

KW - Mouse

KW - Opioid μ-receptor

KW - Radioligand binding

KW - Receptor phosphorylation

KW - Sinomenine

KW - Transfected cell

UR - http://www.scopus.com/inward/record.url?scp=50249107800&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=50249107800&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2008.07.088

DO - 10.1016/j.neulet.2008.07.088

M3 - Article

VL - 443

SP - 209

EP - 212

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -