Activation of extracellular signal-regulated kinase (ERK)1/2, but not p38 and c-Jun N-terminal kinase, is involved in signaling of a novel cytokine, ML-1

Mio Kawaguchi, Luiz F. Onuchic, Shau Ku Huang

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

A novel cytokine, ML-1, was recently discovered, which shares a similar sequence homology with, but is functionally distinct from, IL-17 (Kawaguchi, M., Onuchic, L., Li, X. D., Essayan, D. M., Schroeder, J., Xiao, H. Q., Liu, M. C., Krishnaswamy, G., Germino, G., and Huang, S. K. (2001) J. Immunol. 167, 4430-4435). To determine the signaling mechanisms of ML-1, we investigated activation of mitogen-activated protein (MAP) kinases induced by ML-1. Results show that ML-1 induces in a time-dependent fashion the expression of IL-6 and IL-8 in both primary bronchial epithelial cells (PBECs) and human umbilical vein endothelial cells (HUVECs). ML-1 activated a MAP kinase and an extracellular signal-regulated kinase (ERK)1/2 but not p38 or the c-Jun N-terminal kinase (JNK) in both cell types. Selective MAP kinase kinase (MEK)1/2 inhibitors, PD98059 and U0126, inhibited, in a dose-dependent manner, ML-1-induced expression of IL-6 and IL-8. These findings suggest that ML-1-induced IL-6 and IL-8 production is mediated through the activation of ERK1/2 in both cell types.

Original languageEnglish
Pages (from-to)15229-15232
Number of pages4
JournalJournal of Biological Chemistry
Volume277
Issue number18
DOIs
Publication statusPublished - May 3 2002
Externally publishedYes

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Mitogen-Activated Protein Kinase 3
Interleukin-17
JNK Mitogen-Activated Protein Kinases
Mitogen-Activated Protein Kinase 1
Interleukin-8
Interleukin-6
Chemical activation
Mitogen-Activated Protein Kinases
MAP Kinase Kinase 2
MAP Kinase Kinase 1
Endothelial cells
Human Umbilical Vein Endothelial Cells
Sequence Homology
Epithelial Cells

ASJC Scopus subject areas

  • Biochemistry

Cite this

Activation of extracellular signal-regulated kinase (ERK)1/2, but not p38 and c-Jun N-terminal kinase, is involved in signaling of a novel cytokine, ML-1. / Kawaguchi, Mio; Onuchic, Luiz F.; Huang, Shau Ku.

In: Journal of Biological Chemistry, Vol. 277, No. 18, 03.05.2002, p. 15229-15232.

Research output: Contribution to journalArticle

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