Activation of estrogen receptors with E2 downregulates peroxisome proliferator-activated receptor γ in hepatocellular carcinoma

Yueh Min Lin, Bharath Kumar Velmurugan, Yu Lan Yeh, Chuan Chou Tu, Tsung Jung Ho, Tung-Iuan Lai, Chih Hao Tsai, Fuu Jen Tsai, Chang Hai Tsai, Chih Yang Huang

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17 Citations (Scopus)

Abstract

Hepatocellular carcinoma (HCC) is a leading cause of cancer-related mortality and occurs more often in men than in women; however, little is known about its underlying molecular mechanisms. The present study investigated the effect of estrogen receptor (ER)α and ERβ on peroxisome proliferator-activated receptor γ (PPARγ) expression in Hep3B cells. We examined PPARγ, ERα and ERβ mRNA and protein expression by RT-PCR and western blotting. In order to determine whether PPARγ plays a central role in HCC, we screened for PPARγ expression in liver cancer patient tissues and differentially differentiated HCC cell lines (HA22T, Huh-7, Hep3B and HepG2). We found that PPARγ expression was highly expressed in liver cancer tissues and in Hep3B cells. Furthermore, overexpression of ERα and ERβ was found to decrease PPARγ expression at the transcriptional as well as at the translational level in a ligand-dependent manner. In summary, the present study demonstrated that both ERα and β were sufficient to inhibit PPARγ and provide a valuable therapeutic option for the treatment of HCC patients.

Original languageEnglish
Pages (from-to)3027-3031
Number of pages5
JournalOncology Reports
Volume30
Issue number6
DOIs
Publication statusPublished - Dec 2013

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Keywords

  • Estrogen receptor α
  • Estrogen receptor β
  • Hepatocellular carcinoma
  • Nuclear receptors
  • Peroxisome proliferator-activated receptor γ

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Lin, Y. M., Velmurugan, B. K., Yeh, Y. L., Tu, C. C., Ho, T. J., Lai, T-I., Tsai, C. H., Tsai, F. J., Tsai, C. H., & Huang, C. Y. (2013). Activation of estrogen receptors with E2 downregulates peroxisome proliferator-activated receptor γ in hepatocellular carcinoma. Oncology Reports, 30(6), 3027-3031. https://doi.org/10.3892/or.2013.2793