Activation of c-Jun NH2-terminal kinase and subsequent CPP32/Yama during topoisomerase inhibitor β-lapachone-induced apoptosis through an oxidation-dependent pathway

Shine Gwo Shiah, Shuang En Chuang, Yat Pang Chau, Shing Chuan Shen, Min Liang Kuo

Research output: Contribution to journalArticle

87 Citations (Scopus)

Abstract

β-Lapachone (β-Lap) has been found to inhibit DNA topoisomerases (Topos) by a mechanism distinct from that of other commonly known Topo inhibitors. Here, we demonstrated a pronounced elevation of H2O2 and O2 - in human leukemia HL-60 cells treated with β-Lap. Treatment with other Topo poisons, such as camptothecin (CPT), VP-16, and GL331, did not have the same effect. On the other hand, antioxidant vitamin C (Vit C) treatment effectively antagonized β-Lap-induced apoptosis. This suggested that a reactive oxygen species (ROS)-related pathway was involved in β-Lap-induced apoptosis program. We also found that c-Jun NH2-terminal kinase (JNK) but not p38 mitogen-activated protein kinase or extracellular signal-regulated kinase 1/2 was persistently activated in apoptosis induced by β-Lap. Overexpression of a dominant-negative mutant mitogen-activated protein kinase 1 (MEKK1-DN) or treatment with JNK-specific antisense oligonucleotide or Vit C all prevented β-Lap-induced JNK activation and the subsequent apoptosis. Only the expression of MEKK1-DN, not Vit C treatment, blocked the JNK activity induced by CPT, VP-16, or GL331. These results confirm again that ROS acts as a mediator for JNK activation during β-Lap-induced apoptosis. Furthermore, we found that β-Lap can stimulate CPP32/Yama activity, which was, however, markedly inhibited by the MEKK1-DN expression or Vit C treatment. Again, CPT-induced CPP32/Yama activation can be abolished by MEKK1-DN but not by Vit C treatment. Taken together, these results indicate that β-Lap but not other Topo inhibitors triggers apoptosis signaling, i.e., JNK and subsequent CPP32/Yama activation are mediated by the generation of ROS.

Original languageEnglish
Pages (from-to)391-398
Number of pages8
JournalCancer Research
Volume59
Issue number2
Publication statusPublished - Jan 16 1999
Externally publishedYes

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

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