Activation and up-regulation of nitric oxide synthase in human umbilical vein endothelial cells by polycyclic aromatic hydrocarbons

Ching H. Li, Chen Chen Lee, Yu W. Cheng, Hwe A. Juang, Jaw J. Kang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

PAHs, including naphthalene, fluoranthene and fluorene rapidly induced extracellular Ca2+ influx and hence elevation of intracellular Ca2+ concentration and NO production. The effect can be inhibited by Ca2+ channel blocker but not by P450 inhibitor. In addition to the rapid effect, we have also found that the eNOS mRNA and protein expression were augmented in HUVECs treated with PAHs at concentration as low as 0.1 μM for 24 h. These effects were abolished when the HUVECs were pretreated with the BAPTA, NiCl2 and SKF96365, as well as SKF525A. Our results revealed that, for the first time, PAHs induce the activation of eNOS and enhance eNOS protein expression in HUVECs both in a Ca2+-dependent manner.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalToxicology Letters
Volume151
Issue number2
DOIs
Publication statusPublished - Jul 15 2004

Fingerprint

1-(2-(3-(4-methoxyphenyl)propoxy)-4-methoxyphenylethyl)-1H-imidazole
Polycyclic Aromatic Hydrocarbons
Endothelial cells
Human Umbilical Vein Endothelial Cells
Polycyclic aromatic hydrocarbons
Nitric Oxide Synthase
Up-Regulation
Chemical activation
Proteins
Messenger RNA
fluoranthene
fluorene
naphthalene
1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid

Keywords

  • Ca
  • endothelial nitric oxide synthase
  • Endothelium
  • eNOS
  • human umbilical vein endothelial cells
  • HUVECs
  • NO
  • PAHs
  • Polycyclic aromatic hydrocarbons
  • polycyclic aromatic hydrocarbons

ASJC Scopus subject areas

  • Toxicology

Cite this

Activation and up-regulation of nitric oxide synthase in human umbilical vein endothelial cells by polycyclic aromatic hydrocarbons. / Li, Ching H.; Lee, Chen Chen; Cheng, Yu W.; Juang, Hwe A.; Kang, Jaw J.

In: Toxicology Letters, Vol. 151, No. 2, 15.07.2004, p. 367-374.

Research output: Contribution to journalArticle

@article{b63ee284b4504781895a85084b581b94,
title = "Activation and up-regulation of nitric oxide synthase in human umbilical vein endothelial cells by polycyclic aromatic hydrocarbons",
abstract = "PAHs, including naphthalene, fluoranthene and fluorene rapidly induced extracellular Ca2+ influx and hence elevation of intracellular Ca2+ concentration and NO production. The effect can be inhibited by Ca2+ channel blocker but not by P450 inhibitor. In addition to the rapid effect, we have also found that the eNOS mRNA and protein expression were augmented in HUVECs treated with PAHs at concentration as low as 0.1 μM for 24 h. These effects were abolished when the HUVECs were pretreated with the BAPTA, NiCl2 and SKF96365, as well as SKF525A. Our results revealed that, for the first time, PAHs induce the activation of eNOS and enhance eNOS protein expression in HUVECs both in a Ca2+-dependent manner.",
keywords = "Ca, endothelial nitric oxide synthase, Endothelium, eNOS, human umbilical vein endothelial cells, HUVECs, NO, PAHs, Polycyclic aromatic hydrocarbons, polycyclic aromatic hydrocarbons",
author = "Li, {Ching H.} and Lee, {Chen Chen} and Cheng, {Yu W.} and Juang, {Hwe A.} and Kang, {Jaw J.}",
year = "2004",
month = "7",
day = "15",
doi = "10.1016/j.toxlet.2004.03.003",
language = "English",
volume = "151",
pages = "367--374",
journal = "Toxicology Letters",
issn = "0378-4274",
publisher = "Elsevier BV",
number = "2",

}

TY - JOUR

T1 - Activation and up-regulation of nitric oxide synthase in human umbilical vein endothelial cells by polycyclic aromatic hydrocarbons

AU - Li, Ching H.

AU - Lee, Chen Chen

AU - Cheng, Yu W.

AU - Juang, Hwe A.

AU - Kang, Jaw J.

PY - 2004/7/15

Y1 - 2004/7/15

N2 - PAHs, including naphthalene, fluoranthene and fluorene rapidly induced extracellular Ca2+ influx and hence elevation of intracellular Ca2+ concentration and NO production. The effect can be inhibited by Ca2+ channel blocker but not by P450 inhibitor. In addition to the rapid effect, we have also found that the eNOS mRNA and protein expression were augmented in HUVECs treated with PAHs at concentration as low as 0.1 μM for 24 h. These effects were abolished when the HUVECs were pretreated with the BAPTA, NiCl2 and SKF96365, as well as SKF525A. Our results revealed that, for the first time, PAHs induce the activation of eNOS and enhance eNOS protein expression in HUVECs both in a Ca2+-dependent manner.

AB - PAHs, including naphthalene, fluoranthene and fluorene rapidly induced extracellular Ca2+ influx and hence elevation of intracellular Ca2+ concentration and NO production. The effect can be inhibited by Ca2+ channel blocker but not by P450 inhibitor. In addition to the rapid effect, we have also found that the eNOS mRNA and protein expression were augmented in HUVECs treated with PAHs at concentration as low as 0.1 μM for 24 h. These effects were abolished when the HUVECs were pretreated with the BAPTA, NiCl2 and SKF96365, as well as SKF525A. Our results revealed that, for the first time, PAHs induce the activation of eNOS and enhance eNOS protein expression in HUVECs both in a Ca2+-dependent manner.

KW - Ca

KW - endothelial nitric oxide synthase

KW - Endothelium

KW - eNOS

KW - human umbilical vein endothelial cells

KW - HUVECs

KW - NO

KW - PAHs

KW - Polycyclic aromatic hydrocarbons

KW - polycyclic aromatic hydrocarbons

UR - http://www.scopus.com/inward/record.url?scp=2942616784&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=2942616784&partnerID=8YFLogxK

U2 - 10.1016/j.toxlet.2004.03.003

DO - 10.1016/j.toxlet.2004.03.003

M3 - Article

C2 - 15183461

AN - SCOPUS:2942616784

VL - 151

SP - 367

EP - 374

JO - Toxicology Letters

JF - Toxicology Letters

SN - 0378-4274

IS - 2

ER -