Activation and up-regulation of nitric oxide synthase in human umbilical vein endothelial cells by polycyclic aromatic hydrocarbons

Ching H. Li, Chen Chen Lee, Yu W. Cheng, Hwe A. Juang, Jaw J. Kang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

PAHs, including naphthalene, fluoranthene and fluorene rapidly induced extracellular Ca2+ influx and hence elevation of intracellular Ca2+ concentration and NO production. The effect can be inhibited by Ca2+ channel blocker but not by P450 inhibitor. In addition to the rapid effect, we have also found that the eNOS mRNA and protein expression were augmented in HUVECs treated with PAHs at concentration as low as 0.1 μM for 24 h. These effects were abolished when the HUVECs were pretreated with the BAPTA, NiCl2 and SKF96365, as well as SKF525A. Our results revealed that, for the first time, PAHs induce the activation of eNOS and enhance eNOS protein expression in HUVECs both in a Ca2+-dependent manner.

Original languageEnglish
Pages (from-to)367-374
Number of pages8
JournalToxicology Letters
Volume151
Issue number2
DOIs
Publication statusPublished - Jul 15 2004

Keywords

  • Ca
  • endothelial nitric oxide synthase
  • Endothelium
  • eNOS
  • human umbilical vein endothelial cells
  • HUVECs
  • NO
  • PAHs
  • Polycyclic aromatic hydrocarbons
  • polycyclic aromatic hydrocarbons

ASJC Scopus subject areas

  • Toxicology

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