Activated type I phosphatidylinositol kinase is associated with the epidermal growth factor (EGF) receptor following EGF stimulation

Jeffrey D. Bjorge, Tung On Chan, Michael Antczak, Hsing Jien Kung, Donald J. Fujita

Research output: Contribution to journalArticle

124 Citations (Scopus)

Abstract

We have shown that a type I phosphatidylinositol (PI) kinase activity is associated with the epidermal growth factor (EGF) receptor in a mouse fibroblast cell line expressing human EGF receptors (NRHER5) and that this activity increases dramatically upon treatment of cells with physiologically relevant concentrations of EGF. EGF stimulated a time-dependent increase in EGF receptor-associated PI kinase activity measured in EGF receptor immunoprecipitates. Activation was detected 15 min after the addition of EGF, and it peaked between 1 and 2 hr. Activation of PI kinase was detected with EGF concentrations as low as 10 pM and maximal stimulation occurred at ≈1 nM. Analysis of deacylated PI phosphate products, and inhibition of the PI kinase activity by nonionic detergent, indicated that the PI kinase described here was type I or PI 3′ kinase. These results demonstrate the regulation of a type I PI kinase by EGF and suggest a potential role in the EGF receptor signal transduction pathway. (.

Original languageEnglish
Pages (from-to)3816-3820
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number10
DOIs
Publication statusPublished - Jan 1 1990
Externally publishedYes

Fingerprint

Phosphatidylinositols
Epidermal Growth Factor Receptor
Epidermal Growth Factor
Phosphotransferases
Phosphatidylinositol 3-Kinase
Phosphatidylinositol Phosphates
Detergents
Signal Transduction
Fibroblasts
Cell Line

Keywords

  • Signal transduction
  • Tyrosine kinase

ASJC Scopus subject areas

  • General

Cite this

Activated type I phosphatidylinositol kinase is associated with the epidermal growth factor (EGF) receptor following EGF stimulation. / Bjorge, Jeffrey D.; Chan, Tung On; Antczak, Michael; Kung, Hsing Jien; Fujita, Donald J.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 10, 01.01.1990, p. 3816-3820.

Research output: Contribution to journalArticle

@article{11a513b025e541409d2a8456c8f6c5bb,
title = "Activated type I phosphatidylinositol kinase is associated with the epidermal growth factor (EGF) receptor following EGF stimulation",
abstract = "We have shown that a type I phosphatidylinositol (PI) kinase activity is associated with the epidermal growth factor (EGF) receptor in a mouse fibroblast cell line expressing human EGF receptors (NRHER5) and that this activity increases dramatically upon treatment of cells with physiologically relevant concentrations of EGF. EGF stimulated a time-dependent increase in EGF receptor-associated PI kinase activity measured in EGF receptor immunoprecipitates. Activation was detected 15 min after the addition of EGF, and it peaked between 1 and 2 hr. Activation of PI kinase was detected with EGF concentrations as low as 10 pM and maximal stimulation occurred at ≈1 nM. Analysis of deacylated PI phosphate products, and inhibition of the PI kinase activity by nonionic detergent, indicated that the PI kinase described here was type I or PI 3′ kinase. These results demonstrate the regulation of a type I PI kinase by EGF and suggest a potential role in the EGF receptor signal transduction pathway. (.",
keywords = "Signal transduction, Tyrosine kinase",
author = "Bjorge, {Jeffrey D.} and Chan, {Tung On} and Michael Antczak and Kung, {Hsing Jien} and Fujita, {Donald J.}",
year = "1990",
month = "1",
day = "1",
doi = "10.1073/pnas.87.10.3816",
language = "English",
volume = "87",
pages = "3816--3820",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "10",

}

TY - JOUR

T1 - Activated type I phosphatidylinositol kinase is associated with the epidermal growth factor (EGF) receptor following EGF stimulation

AU - Bjorge, Jeffrey D.

AU - Chan, Tung On

AU - Antczak, Michael

AU - Kung, Hsing Jien

AU - Fujita, Donald J.

PY - 1990/1/1

Y1 - 1990/1/1

N2 - We have shown that a type I phosphatidylinositol (PI) kinase activity is associated with the epidermal growth factor (EGF) receptor in a mouse fibroblast cell line expressing human EGF receptors (NRHER5) and that this activity increases dramatically upon treatment of cells with physiologically relevant concentrations of EGF. EGF stimulated a time-dependent increase in EGF receptor-associated PI kinase activity measured in EGF receptor immunoprecipitates. Activation was detected 15 min after the addition of EGF, and it peaked between 1 and 2 hr. Activation of PI kinase was detected with EGF concentrations as low as 10 pM and maximal stimulation occurred at ≈1 nM. Analysis of deacylated PI phosphate products, and inhibition of the PI kinase activity by nonionic detergent, indicated that the PI kinase described here was type I or PI 3′ kinase. These results demonstrate the regulation of a type I PI kinase by EGF and suggest a potential role in the EGF receptor signal transduction pathway. (.

AB - We have shown that a type I phosphatidylinositol (PI) kinase activity is associated with the epidermal growth factor (EGF) receptor in a mouse fibroblast cell line expressing human EGF receptors (NRHER5) and that this activity increases dramatically upon treatment of cells with physiologically relevant concentrations of EGF. EGF stimulated a time-dependent increase in EGF receptor-associated PI kinase activity measured in EGF receptor immunoprecipitates. Activation was detected 15 min after the addition of EGF, and it peaked between 1 and 2 hr. Activation of PI kinase was detected with EGF concentrations as low as 10 pM and maximal stimulation occurred at ≈1 nM. Analysis of deacylated PI phosphate products, and inhibition of the PI kinase activity by nonionic detergent, indicated that the PI kinase described here was type I or PI 3′ kinase. These results demonstrate the regulation of a type I PI kinase by EGF and suggest a potential role in the EGF receptor signal transduction pathway. (.

KW - Signal transduction

KW - Tyrosine kinase

UR - http://www.scopus.com/inward/record.url?scp=0025316902&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025316902&partnerID=8YFLogxK

U2 - 10.1073/pnas.87.10.3816

DO - 10.1073/pnas.87.10.3816

M3 - Article

VL - 87

SP - 3816

EP - 3820

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 10

ER -