Actinobacillus pleuropneumoniae serotype 10 derived ApxI induces apoptosis in porcine alveolar macrophages

Maw Sheng Chien, You Yu Chan, Zeng Weng Chen, Chi Ming Wu, Jiunn Wang Liao, Ter Hsin Chen, Wei Cheng Lee, Kuang Sheng Yeh, Shih Ling Hsuan

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Actinobacillus pleuropneumoniae (AP) is the causative agent of swine pleuropneumonia, a fibrinous, exudative, hemorrhagic, necrotizing pleuropneumonia affecting all ages of pigs. Actinobacillus pleuropneumoniae exotoxins (Apx) are one of the major virulence factors of AP. Due to the complex nature of Apx toxins produced by AP, little is known regarding the interactions of individual species of Apx toxin with target cells. The objective of this study was to examine whether AP serotype 10-derived exotoxin, ApxI, caused apoptosis in porcine alveolar macrophages (PAMs) and to delineate the underlying signaling pathways. Isolated PAMs were stimulated with different concentrations of native ApxI and monitored for apoptosis using Hoechst staining, TUNEL, and DNA laddering assays. The ApxI-stimulated PAMs exhibited typical morphological features of apoptosis, including condensation of chromatin, formation of apoptotic bodies and DNA laddering. ApxI-induced apoptosis in a concentration- and time-dependent manner. Furthermore, to delineate the signaling events involved in ApxI-induced apoptosis, it was observed that caspase 3 was activated in ApxI-stimulated PAMs. Ablation of caspase 3 activity via specific inhibitors protected PAMs from apoptosis by ApxI. This study is the first to demonstrate that native ApxI causes apoptosis in PAMs at low concentrations and that these apoptotic events are mediated via a caspase 3-dependent pathway. These findings suggest a role of ApxI in AP infection as it might impair the host defense system through the induction of apoptosis in PAMs.

Original languageEnglish
Pages (from-to)327-333
Number of pages7
JournalVeterinary Microbiology
Volume135
Issue number3-4
DOIs
Publication statusPublished - Mar 30 2009

Fingerprint

Actinobacillus pleuropneumoniae
Alveolar Macrophages
serotypes
macrophages
Swine
apoptosis
Apoptosis
swine
exotoxins
Exotoxins
caspase-3
Pleuropneumonia
Caspase 3
Actinobacillus Infections
toxins
Serogroup
DNA
In Situ Nick-End Labeling
Virulence Factors
Chromatin

Keywords

  • Actinobacillus pleuropneumoniae
  • Apoptosis
  • ApxI
  • Caspase 3
  • Porcine alveolar macrophages

ASJC Scopus subject areas

  • Microbiology
  • veterinary(all)

Cite this

Chien, M. S., Chan, Y. Y., Chen, Z. W., Wu, C. M., Liao, J. W., Chen, T. H., ... Hsuan, S. L. (2009). Actinobacillus pleuropneumoniae serotype 10 derived ApxI induces apoptosis in porcine alveolar macrophages. Veterinary Microbiology, 135(3-4), 327-333. https://doi.org/10.1016/j.vetmic.2008.09.071

Actinobacillus pleuropneumoniae serotype 10 derived ApxI induces apoptosis in porcine alveolar macrophages. / Chien, Maw Sheng; Chan, You Yu; Chen, Zeng Weng; Wu, Chi Ming; Liao, Jiunn Wang; Chen, Ter Hsin; Lee, Wei Cheng; Yeh, Kuang Sheng; Hsuan, Shih Ling.

In: Veterinary Microbiology, Vol. 135, No. 3-4, 30.03.2009, p. 327-333.

Research output: Contribution to journalArticle

Chien, MS, Chan, YY, Chen, ZW, Wu, CM, Liao, JW, Chen, TH, Lee, WC, Yeh, KS & Hsuan, SL 2009, 'Actinobacillus pleuropneumoniae serotype 10 derived ApxI induces apoptosis in porcine alveolar macrophages', Veterinary Microbiology, vol. 135, no. 3-4, pp. 327-333. https://doi.org/10.1016/j.vetmic.2008.09.071
Chien, Maw Sheng ; Chan, You Yu ; Chen, Zeng Weng ; Wu, Chi Ming ; Liao, Jiunn Wang ; Chen, Ter Hsin ; Lee, Wei Cheng ; Yeh, Kuang Sheng ; Hsuan, Shih Ling. / Actinobacillus pleuropneumoniae serotype 10 derived ApxI induces apoptosis in porcine alveolar macrophages. In: Veterinary Microbiology. 2009 ; Vol. 135, No. 3-4. pp. 327-333.
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abstract = "Actinobacillus pleuropneumoniae (AP) is the causative agent of swine pleuropneumonia, a fibrinous, exudative, hemorrhagic, necrotizing pleuropneumonia affecting all ages of pigs. Actinobacillus pleuropneumoniae exotoxins (Apx) are one of the major virulence factors of AP. Due to the complex nature of Apx toxins produced by AP, little is known regarding the interactions of individual species of Apx toxin with target cells. The objective of this study was to examine whether AP serotype 10-derived exotoxin, ApxI, caused apoptosis in porcine alveolar macrophages (PAMs) and to delineate the underlying signaling pathways. Isolated PAMs were stimulated with different concentrations of native ApxI and monitored for apoptosis using Hoechst staining, TUNEL, and DNA laddering assays. The ApxI-stimulated PAMs exhibited typical morphological features of apoptosis, including condensation of chromatin, formation of apoptotic bodies and DNA laddering. ApxI-induced apoptosis in a concentration- and time-dependent manner. Furthermore, to delineate the signaling events involved in ApxI-induced apoptosis, it was observed that caspase 3 was activated in ApxI-stimulated PAMs. Ablation of caspase 3 activity via specific inhibitors protected PAMs from apoptosis by ApxI. This study is the first to demonstrate that native ApxI causes apoptosis in PAMs at low concentrations and that these apoptotic events are mediated via a caspase 3-dependent pathway. These findings suggest a role of ApxI in AP infection as it might impair the host defense system through the induction of apoptosis in PAMs.",
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