Acidic FGF enhances functional regeneration of adult dorsal roots

Liang Ming Lee, Ming Chao Huang, Tien Y. Chuang, Liang Shong Lee, Henrich Cheng, I. H. Lee

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

It has been well documented that the regeneration of sensory axons severed in the dorsal roots into the spinal cord is largely inhibited in adult mammals. We investigated whether peripheral nerve grafts combined with acidic fibroblast growth factor (aFGF) could induce the regeneration of transected dorsal roots in adult rats, as evaluated by cortical somatosensory evoked potentials (SEPs). Median nerve (forelimb) stimuli produced consistent responses in the primary somatosensory cortex of normal rats, but these were completely eliminated after the transection of cervical 6th - 8th roots. The dorsal root stumps were immediately anastomosed to the cord with intercostal nerve grafts. Subsequently, aFGF in fibrin glue was administered to the grafted area. Four to twenty weeks after rhizotomy, six of the seven rats receiving such reconstruction had recovery of SEPs. The reappearing SEPs typically showed similar waveforms and latencies as normal ones. They were eliminated by retransection of the repaired roots, thus verifying their source as the regenerated roots. We present here substantial evidence that aFGF enhances the functional restoration of cut dorsal roots. Cortical SEPs is considered a useful tool in evaluating such regeneration. These results may offer therapeutic potential in the treatment of dorsal root injuries.

Original languageEnglish
Pages (from-to)1937-1943
Number of pages7
JournalLife Sciences
Volume74
Issue number15
DOIs
Publication statusPublished - Feb 27 2004

Fingerprint

Spinal Nerve Roots
Bioelectric potentials
Fibroblast Growth Factor 1
Somatosensory Evoked Potentials
Regeneration
Rats
Grafts
Fibrin Tissue Adhesive
Mammals
Intercostal Nerves
Rhizotomy
Transplants
Somatosensory Cortex
Forelimb
Median Nerve
Restoration
Peripheral Nerves
Axons
Spinal Cord
Recovery

Keywords

  • Acidic fibroblast growth factor
  • Dorsal root injury
  • Peripheral nerve graft
  • Regeneration
  • Somatosensory evoked potentials

ASJC Scopus subject areas

  • Pharmacology

Cite this

Lee, L. M., Huang, M. C., Chuang, T. Y., Lee, L. S., Cheng, H., & Lee, I. H. (2004). Acidic FGF enhances functional regeneration of adult dorsal roots. Life Sciences, 74(15), 1937-1943. https://doi.org/10.1016/j.lfs.2003.09.043

Acidic FGF enhances functional regeneration of adult dorsal roots. / Lee, Liang Ming; Huang, Ming Chao; Chuang, Tien Y.; Lee, Liang Shong; Cheng, Henrich; Lee, I. H.

In: Life Sciences, Vol. 74, No. 15, 27.02.2004, p. 1937-1943.

Research output: Contribution to journalArticle

Lee, LM, Huang, MC, Chuang, TY, Lee, LS, Cheng, H & Lee, IH 2004, 'Acidic FGF enhances functional regeneration of adult dorsal roots', Life Sciences, vol. 74, no. 15, pp. 1937-1943. https://doi.org/10.1016/j.lfs.2003.09.043
Lee LM, Huang MC, Chuang TY, Lee LS, Cheng H, Lee IH. Acidic FGF enhances functional regeneration of adult dorsal roots. Life Sciences. 2004 Feb 27;74(15):1937-1943. https://doi.org/10.1016/j.lfs.2003.09.043
Lee, Liang Ming ; Huang, Ming Chao ; Chuang, Tien Y. ; Lee, Liang Shong ; Cheng, Henrich ; Lee, I. H. / Acidic FGF enhances functional regeneration of adult dorsal roots. In: Life Sciences. 2004 ; Vol. 74, No. 15. pp. 1937-1943.
@article{26bc3dfb046546e3b263e747db913761,
title = "Acidic FGF enhances functional regeneration of adult dorsal roots",
abstract = "It has been well documented that the regeneration of sensory axons severed in the dorsal roots into the spinal cord is largely inhibited in adult mammals. We investigated whether peripheral nerve grafts combined with acidic fibroblast growth factor (aFGF) could induce the regeneration of transected dorsal roots in adult rats, as evaluated by cortical somatosensory evoked potentials (SEPs). Median nerve (forelimb) stimuli produced consistent responses in the primary somatosensory cortex of normal rats, but these were completely eliminated after the transection of cervical 6th - 8th roots. The dorsal root stumps were immediately anastomosed to the cord with intercostal nerve grafts. Subsequently, aFGF in fibrin glue was administered to the grafted area. Four to twenty weeks after rhizotomy, six of the seven rats receiving such reconstruction had recovery of SEPs. The reappearing SEPs typically showed similar waveforms and latencies as normal ones. They were eliminated by retransection of the repaired roots, thus verifying their source as the regenerated roots. We present here substantial evidence that aFGF enhances the functional restoration of cut dorsal roots. Cortical SEPs is considered a useful tool in evaluating such regeneration. These results may offer therapeutic potential in the treatment of dorsal root injuries.",
keywords = "Acidic fibroblast growth factor, Dorsal root injury, Peripheral nerve graft, Regeneration, Somatosensory evoked potentials",
author = "Lee, {Liang Ming} and Huang, {Ming Chao} and Chuang, {Tien Y.} and Lee, {Liang Shong} and Henrich Cheng and Lee, {I. H.}",
year = "2004",
month = "2",
day = "27",
doi = "10.1016/j.lfs.2003.09.043",
language = "English",
volume = "74",
pages = "1937--1943",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "15",

}

TY - JOUR

T1 - Acidic FGF enhances functional regeneration of adult dorsal roots

AU - Lee, Liang Ming

AU - Huang, Ming Chao

AU - Chuang, Tien Y.

AU - Lee, Liang Shong

AU - Cheng, Henrich

AU - Lee, I. H.

PY - 2004/2/27

Y1 - 2004/2/27

N2 - It has been well documented that the regeneration of sensory axons severed in the dorsal roots into the spinal cord is largely inhibited in adult mammals. We investigated whether peripheral nerve grafts combined with acidic fibroblast growth factor (aFGF) could induce the regeneration of transected dorsal roots in adult rats, as evaluated by cortical somatosensory evoked potentials (SEPs). Median nerve (forelimb) stimuli produced consistent responses in the primary somatosensory cortex of normal rats, but these were completely eliminated after the transection of cervical 6th - 8th roots. The dorsal root stumps were immediately anastomosed to the cord with intercostal nerve grafts. Subsequently, aFGF in fibrin glue was administered to the grafted area. Four to twenty weeks after rhizotomy, six of the seven rats receiving such reconstruction had recovery of SEPs. The reappearing SEPs typically showed similar waveforms and latencies as normal ones. They were eliminated by retransection of the repaired roots, thus verifying their source as the regenerated roots. We present here substantial evidence that aFGF enhances the functional restoration of cut dorsal roots. Cortical SEPs is considered a useful tool in evaluating such regeneration. These results may offer therapeutic potential in the treatment of dorsal root injuries.

AB - It has been well documented that the regeneration of sensory axons severed in the dorsal roots into the spinal cord is largely inhibited in adult mammals. We investigated whether peripheral nerve grafts combined with acidic fibroblast growth factor (aFGF) could induce the regeneration of transected dorsal roots in adult rats, as evaluated by cortical somatosensory evoked potentials (SEPs). Median nerve (forelimb) stimuli produced consistent responses in the primary somatosensory cortex of normal rats, but these were completely eliminated after the transection of cervical 6th - 8th roots. The dorsal root stumps were immediately anastomosed to the cord with intercostal nerve grafts. Subsequently, aFGF in fibrin glue was administered to the grafted area. Four to twenty weeks after rhizotomy, six of the seven rats receiving such reconstruction had recovery of SEPs. The reappearing SEPs typically showed similar waveforms and latencies as normal ones. They were eliminated by retransection of the repaired roots, thus verifying their source as the regenerated roots. We present here substantial evidence that aFGF enhances the functional restoration of cut dorsal roots. Cortical SEPs is considered a useful tool in evaluating such regeneration. These results may offer therapeutic potential in the treatment of dorsal root injuries.

KW - Acidic fibroblast growth factor

KW - Dorsal root injury

KW - Peripheral nerve graft

KW - Regeneration

KW - Somatosensory evoked potentials

UR - http://www.scopus.com/inward/record.url?scp=0842346120&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0842346120&partnerID=8YFLogxK

U2 - 10.1016/j.lfs.2003.09.043

DO - 10.1016/j.lfs.2003.09.043

M3 - Article

VL - 74

SP - 1937

EP - 1943

JO - Life Sciences

JF - Life Sciences

SN - 0024-3205

IS - 15

ER -