Accumulation of methylglyoxal and d-lactate in Pb-induced nephrotoxicity in rats

Yu Shen Huang, Yi Chieh Li, Pei Yun Tsai, Chia En Lin, Chien Ming Chen, Shih-Ming Chen, Jen-Ai Lee

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Lead (Pb) is an environmental pollutant associated with several diseases, such as nephrotoxicity. Methylglyoxal (MG) is a reactive dicarbonyl compound formed during glycolysis and reported to increase in kidney damage. Metformin is used as an MG scavenger in the clinic. In this study, we investigated the mechanism of Pb-induced renal injury and the effect of metformin on Pb-induced nephrotoxicity. Eighteen Wistar rats were randomly divided into three groups: control, Pb, and Pb+metformin groups. Pb (250ppm) was administered in drinking water, and 50mg/kg of metformin was co-administered orally. After 28days, the levels of MG and its metabolite d-lactate in urine, serum and renal tissues were examined. The elevation of renal MG (56.86±17.47 vs 36.40±5.69, p<0.01) and urinary d-lactate (0.68±0.28 vs 0.32±0.13, p<0.01) was observed in Pb-exposed rats compared with those in control rats. After co-treatment with metformin, these phenomena were attenuated. In the present study, it was demonstrated for the first time that urinary d-lactate might serve as the candidate marker for Pb-induced nephrotoxicity in the clinic, and metformin might be a new therapeutic candidate for Pb poisoning.

Original languageEnglish
JournalBiomedical Chromatography
DOIs
Publication statusPublished - May 2017

Fingerprint

Pyruvaldehyde
Metformin
Rats
Lactic Acid
Kidney
Rat control
Environmental Pollutants
Glycolysis
Metabolites
Drinking Water
Poisoning
Wistar Rats
Urine
Tissue
Control Groups
Wounds and Injuries
Serum

Keywords

  • d-lactate
  • Heavy metal
  • Methylglyoxal
  • Nephrotoxicity
  • Pb

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Molecular Biology
  • Pharmacology
  • Drug Discovery
  • Clinical Biochemistry

Cite this

Accumulation of methylglyoxal and d-lactate in Pb-induced nephrotoxicity in rats. / Huang, Yu Shen; Li, Yi Chieh; Tsai, Pei Yun; Lin, Chia En; Chen, Chien Ming; Chen, Shih-Ming; Lee, Jen-Ai.

In: Biomedical Chromatography, 05.2017.

Research output: Contribution to journalArticle

Huang, Yu Shen ; Li, Yi Chieh ; Tsai, Pei Yun ; Lin, Chia En ; Chen, Chien Ming ; Chen, Shih-Ming ; Lee, Jen-Ai. / Accumulation of methylglyoxal and d-lactate in Pb-induced nephrotoxicity in rats. In: Biomedical Chromatography. 2017.
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