Accelerated bioethanol fermentation by using a novel yeast immobilization technique

Microtube array membrane

Chien Chung Chen, Chien Hui Wu, Jhih Jhong Wu, Chien Chih Chiu, Chien Hsuan Wong, Min Lang Tsai, Hong Ting Victor Lin

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Cell immobilization is a way to isolate or localize intact cells in a certain space and maintain their catalytic activity. Immobilized cells can effectively reduce the negative effects of inhibitors and the processing cost of inoculum preparation for continuous or fed-batch fermentation of microorganisms. In this study, a novel yeast immobilization technique using renewable poly-l-lactic acid (PLLA) microtube array membrane (MTAM) was thoroughly evaluated for bioethanol fermentation. PLLA-MTAM was shown to be stable in 15% (v/v) ethanol solution during shaking cultivation. A yeast encapsulation efficiency of 67-70% was obtained, and the yeasts in MTAMs with greater porosity showed greater bioethanol productivity. The MTAM-immobilized Kluyveromyces marxianus, prepared using in situ and siphon methods, were evaluated using 5% (w/v) glucose fermentation. Improved glucose consumption and bioethanol production were observed in batch bioethanol fermentation. In 7 cycles during repeated-batch fermentation, the immobilized yeasts prepared using the in situ method showed a maximum CEtOH of 24.23 g/L, maximum YP/S of 0.48 g/g, and r PEtOH of 2.69 g/L h. Our data indicated that the PLLA-MTAM immobilized yeasts significantly enhanced bioethanol productivity and was a novel, promising technology for bioethanol fermentation.

Original languageEnglish
Article number10450
Pages (from-to)1509-1515
Number of pages7
JournalProcess Biochemistry
Volume50
Issue number10
DOIs
Publication statusPublished - Oct 1 2015

Fingerprint

Bioethanol
Immobilization
Yeast
Fermentation
Yeasts
Membranes
Lactic acid
Lactic Acid
Glucose
Cell immobilization
Productivity
Kluyveromyces
Siphons
Immobilized Cells
Porosity
Encapsulation
Microorganisms
Catalyst activity
Ethanol
Cells

Keywords

  • Bioethanol
  • Fermentation
  • Kluyveromyces marxianus
  • Microtube array membrane (MTAM)
  • Repeated-batch

ASJC Scopus subject areas

  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Bioengineering

Cite this

Accelerated bioethanol fermentation by using a novel yeast immobilization technique : Microtube array membrane. / Chen, Chien Chung; Wu, Chien Hui; Wu, Jhih Jhong; Chiu, Chien Chih; Wong, Chien Hsuan; Tsai, Min Lang; Lin, Hong Ting Victor.

In: Process Biochemistry, Vol. 50, No. 10, 10450, 01.10.2015, p. 1509-1515.

Research output: Contribution to journalArticle

Chen, Chien Chung ; Wu, Chien Hui ; Wu, Jhih Jhong ; Chiu, Chien Chih ; Wong, Chien Hsuan ; Tsai, Min Lang ; Lin, Hong Ting Victor. / Accelerated bioethanol fermentation by using a novel yeast immobilization technique : Microtube array membrane. In: Process Biochemistry. 2015 ; Vol. 50, No. 10. pp. 1509-1515.
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