Aberrant expression and distribution of the OCT-4 transcription factor in seminomas

Chien Jui Cheng, Yu Chih Wu, Jye An Shu, Thai Yen Ling, Hung Chih Kuo, Jui Yu Wu, E-E Chang, Shyh Chern Chang, Yen Hua Huang

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Testicular germ cell tumors (TGCTs), comprised of seminomas and non-seminomas, are derived from premalignant and noninvasive intracellular germ cell neoplasias. Among TGCTs, seminomas are believed to resemble a transformed state of primordial germ cells (PGCs) and are known to exhibit a gene expression profile similar to that of embryonic stem (ES) cells, such as transcription factor OCT-4. OCT-4 has recently been recognized as a diagnostic marker for clinical aspects of seminomas. However, the role of the OCT-4 protein in seminomas has not been clarified. To determine a possible role of the OCT-4 protein in seminomas, in this paper, we studied a series of 41 testicular tumor tissues and four cell lines by immunohistochemistry, Western blotting, and reverse-transcriptase polymerase chain reaction (RT-PCR) to examine the expression and distribution of the OCT-4 transcription factor in seminomas. By utilizing immunohistochemical staining and Western blotting, we demonstrated that the OCT-4 transcription factor was aberrantly localized in the cytoplasm and nuclei of cells in the collected seminoma tissues. This observation was further confirmed using immunocytochemical staining of NCCIT (seminoma-embryonal carcinoma) and NT2 (embryonal carcinoma) cells. In addition, the RT-PCR results indicated that Oct-4 mRNA was relatively highly expressed in NCCIT, NT2 cells, and seminoma tissues when compared with human embryonic stem cells. The aberrant expression and distribution of the OCT-4 transcription factor in seminomas may provide some important clues concerning the cell transformation between germ line stem cells (like PGC) and testicular germ cell tumors.

Original languageEnglish
Pages (from-to)797-807
Number of pages11
JournalJournal of Biomedical Science
Volume14
Issue number6
DOIs
Publication statusPublished - Nov 2007

Fingerprint

Seminoma
Transcription Factors
Cells
Tumors
Stem cells
Polymerase chain reaction
RNA-Directed DNA Polymerase
Tissue
Germ Cells
Reverse Transcriptase Polymerase Chain Reaction
Gene expression
Proteins
Western Blotting
Embryonal Carcinoma
Staining and Labeling
Embryonal Carcinoma Stem Cells
Messenger RNA
Testicular Neoplasms
Embryonic Stem Cells
Cell Nucleus

Keywords

  • Cytosolic distribution
  • OCT-4 transcription factor
  • Seminoma
  • Testicular germ cell tumors

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Aberrant expression and distribution of the OCT-4 transcription factor in seminomas. / Cheng, Chien Jui; Wu, Yu Chih; Shu, Jye An; Ling, Thai Yen; Kuo, Hung Chih; Wu, Jui Yu; Chang, E-E; Chang, Shyh Chern; Huang, Yen Hua.

In: Journal of Biomedical Science, Vol. 14, No. 6, 11.2007, p. 797-807.

Research output: Contribution to journalArticle

Cheng, Chien Jui ; Wu, Yu Chih ; Shu, Jye An ; Ling, Thai Yen ; Kuo, Hung Chih ; Wu, Jui Yu ; Chang, E-E ; Chang, Shyh Chern ; Huang, Yen Hua. / Aberrant expression and distribution of the OCT-4 transcription factor in seminomas. In: Journal of Biomedical Science. 2007 ; Vol. 14, No. 6. pp. 797-807.
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AU - Chang, Shyh Chern

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AB - Testicular germ cell tumors (TGCTs), comprised of seminomas and non-seminomas, are derived from premalignant and noninvasive intracellular germ cell neoplasias. Among TGCTs, seminomas are believed to resemble a transformed state of primordial germ cells (PGCs) and are known to exhibit a gene expression profile similar to that of embryonic stem (ES) cells, such as transcription factor OCT-4. OCT-4 has recently been recognized as a diagnostic marker for clinical aspects of seminomas. However, the role of the OCT-4 protein in seminomas has not been clarified. To determine a possible role of the OCT-4 protein in seminomas, in this paper, we studied a series of 41 testicular tumor tissues and four cell lines by immunohistochemistry, Western blotting, and reverse-transcriptase polymerase chain reaction (RT-PCR) to examine the expression and distribution of the OCT-4 transcription factor in seminomas. By utilizing immunohistochemical staining and Western blotting, we demonstrated that the OCT-4 transcription factor was aberrantly localized in the cytoplasm and nuclei of cells in the collected seminoma tissues. This observation was further confirmed using immunocytochemical staining of NCCIT (seminoma-embryonal carcinoma) and NT2 (embryonal carcinoma) cells. In addition, the RT-PCR results indicated that Oct-4 mRNA was relatively highly expressed in NCCIT, NT2 cells, and seminoma tissues when compared with human embryonic stem cells. The aberrant expression and distribution of the OCT-4 transcription factor in seminomas may provide some important clues concerning the cell transformation between germ line stem cells (like PGC) and testicular germ cell tumors.

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