Abstract
Testicular germ cell tumors (TGCTs), comprised of seminomas and non-seminomas, are derived from premalignant and noninvasive intracellular germ cell neoplasias. Among TGCTs, seminomas are believed to resemble a transformed state of primordial germ cells (PGCs) and are known to exhibit a gene expression profile similar to that of embryonic stem (ES) cells, such as transcription factor OCT-4. OCT-4 has recently been recognized as a diagnostic marker for clinical aspects of seminomas. However, the role of the OCT-4 protein in seminomas has not been clarified. To determine a possible role of the OCT-4 protein in seminomas, in this paper, we studied a series of 41 testicular tumor tissues and four cell lines by immunohistochemistry, Western blotting, and reverse-transcriptase polymerase chain reaction (RT-PCR) to examine the expression and distribution of the OCT-4 transcription factor in seminomas. By utilizing immunohistochemical staining and Western blotting, we demonstrated that the OCT-4 transcription factor was aberrantly localized in the cytoplasm and nuclei of cells in the collected seminoma tissues. This observation was further confirmed using immunocytochemical staining of NCCIT (seminoma-embryonal carcinoma) and NT2 (embryonal carcinoma) cells. In addition, the RT-PCR results indicated that Oct-4 mRNA was relatively highly expressed in NCCIT, NT2 cells, and seminoma tissues when compared with human embryonic stem cells. The aberrant expression and distribution of the OCT-4 transcription factor in seminomas may provide some important clues concerning the cell transformation between germ line stem cells (like PGC) and testicular germ cell tumors.
| Original language | English |
|---|---|
| Pages (from-to) | 797-807 |
| Number of pages | 11 |
| Journal | Journal of Biomedical Science |
| Volume | 14 |
| Issue number | 6 |
| DOIs | |
| Publication status | Published - Nov 2007 |
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Keywords
- Cytosolic distribution
- OCT-4 transcription factor
- Seminoma
- Testicular germ cell tumors
ASJC Scopus subject areas
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Aberrant expression and distribution of the OCT-4 transcription factor in seminomas. / Cheng, Chien Jui; Wu, Yu Chih; Shu, Jye An; Ling, Thai Yen; Kuo, Hung Chih; Wu, Jui Yu; Chang, E-E; Chang, Shyh Chern; Huang, Yen Hua.
In: Journal of Biomedical Science, Vol. 14, No. 6, 11.2007, p. 797-807.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Aberrant expression and distribution of the OCT-4 transcription factor in seminomas
AU - Cheng, Chien Jui
AU - Wu, Yu Chih
AU - Shu, Jye An
AU - Ling, Thai Yen
AU - Kuo, Hung Chih
AU - Wu, Jui Yu
AU - Chang, E-E
AU - Chang, Shyh Chern
AU - Huang, Yen Hua
PY - 2007/11
Y1 - 2007/11
N2 - Testicular germ cell tumors (TGCTs), comprised of seminomas and non-seminomas, are derived from premalignant and noninvasive intracellular germ cell neoplasias. Among TGCTs, seminomas are believed to resemble a transformed state of primordial germ cells (PGCs) and are known to exhibit a gene expression profile similar to that of embryonic stem (ES) cells, such as transcription factor OCT-4. OCT-4 has recently been recognized as a diagnostic marker for clinical aspects of seminomas. However, the role of the OCT-4 protein in seminomas has not been clarified. To determine a possible role of the OCT-4 protein in seminomas, in this paper, we studied a series of 41 testicular tumor tissues and four cell lines by immunohistochemistry, Western blotting, and reverse-transcriptase polymerase chain reaction (RT-PCR) to examine the expression and distribution of the OCT-4 transcription factor in seminomas. By utilizing immunohistochemical staining and Western blotting, we demonstrated that the OCT-4 transcription factor was aberrantly localized in the cytoplasm and nuclei of cells in the collected seminoma tissues. This observation was further confirmed using immunocytochemical staining of NCCIT (seminoma-embryonal carcinoma) and NT2 (embryonal carcinoma) cells. In addition, the RT-PCR results indicated that Oct-4 mRNA was relatively highly expressed in NCCIT, NT2 cells, and seminoma tissues when compared with human embryonic stem cells. The aberrant expression and distribution of the OCT-4 transcription factor in seminomas may provide some important clues concerning the cell transformation between germ line stem cells (like PGC) and testicular germ cell tumors.
AB - Testicular germ cell tumors (TGCTs), comprised of seminomas and non-seminomas, are derived from premalignant and noninvasive intracellular germ cell neoplasias. Among TGCTs, seminomas are believed to resemble a transformed state of primordial germ cells (PGCs) and are known to exhibit a gene expression profile similar to that of embryonic stem (ES) cells, such as transcription factor OCT-4. OCT-4 has recently been recognized as a diagnostic marker for clinical aspects of seminomas. However, the role of the OCT-4 protein in seminomas has not been clarified. To determine a possible role of the OCT-4 protein in seminomas, in this paper, we studied a series of 41 testicular tumor tissues and four cell lines by immunohistochemistry, Western blotting, and reverse-transcriptase polymerase chain reaction (RT-PCR) to examine the expression and distribution of the OCT-4 transcription factor in seminomas. By utilizing immunohistochemical staining and Western blotting, we demonstrated that the OCT-4 transcription factor was aberrantly localized in the cytoplasm and nuclei of cells in the collected seminoma tissues. This observation was further confirmed using immunocytochemical staining of NCCIT (seminoma-embryonal carcinoma) and NT2 (embryonal carcinoma) cells. In addition, the RT-PCR results indicated that Oct-4 mRNA was relatively highly expressed in NCCIT, NT2 cells, and seminoma tissues when compared with human embryonic stem cells. The aberrant expression and distribution of the OCT-4 transcription factor in seminomas may provide some important clues concerning the cell transformation between germ line stem cells (like PGC) and testicular germ cell tumors.
KW - Cytosolic distribution
KW - OCT-4 transcription factor
KW - Seminoma
KW - Testicular germ cell tumors
UR - http://www.scopus.com/inward/record.url?scp=35348882023&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=35348882023&partnerID=8YFLogxK
U2 - 10.1007/s11373-007-9198-7
DO - 10.1007/s11373-007-9198-7
M3 - Article
C2 - 17682839
AN - SCOPUS:35348882023
VL - 14
SP - 797
EP - 807
JO - Journal of Biomedical Science
JF - Journal of Biomedical Science
SN - 1021-7770
IS - 6
ER -