A whole genome methylation analysis of systemic lupus erythematosus: Hypomethylation of the IL10 and IL1R2 promoters is associated with disease activity

S. Y. Lin, S. C. Hsieh, Y. C. Lin, C. N. Lee, M. H. Tsai, L. C. Lai, E. Y. Chuang, P. C. Chen, C. C. Hung, L. Y. Chen, W. S. Hsieh, D. M. Niu, Y. N. Su, H. N. Ho

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The etiology of systemic lupus erythematosus (SLE) involves a complex interaction of genetic and environmental factors. Investigations have shown that environmentally driven epigenetic changes contribute to the etiology of SLE. Here, we hypothesize that aberrant DNA methylation may contribute to the activation of the immune machinery and trigger lupus disease activity. A whole genome methylation array was applied to investigate the DNA methylation changes between 12 pairs of active SLE patients and healthy controls. The results were further confirmed in 66 SLE patients, 102 healthy controls. The methylation statuses of the IL10 and IL1R2 genes were significantly reduced in the SLE patient samples relative to the healthy controls (age-adjusted odds ratios, 64.2 and 16.9, respectively, P<0.0001). There was a trend toward SLE patients having hypomethylated IL10 and IL1R2 genes accompanied by greater disease activity. We observed that the methylation degree of IL10 and IL1R2 genes were reduced in the rheumatoid arthritis (RA) patients as well but the hypomethylation change was more significant in IL1R2 genes than in the IL10 genes in RA patients. This study demonstrated that DNA hypomethylation might be associated with SLE. Hypomethylated IL10 and IL1R2 genes may provide potential epigenetic markers as clinical predictors for autoimmune diseases.

Original languageEnglish
Pages (from-to)214-220
Number of pages7
JournalGenes and Immunity
Volume13
Issue number3
DOIs
Publication statusPublished - Apr 1 2012
Externally publishedYes

Keywords

  • DNA hypomethylation
  • IL10
  • IL1R2
  • methylation array
  • systemic lupus erythematosus

ASJC Scopus subject areas

  • Immunology
  • Genetics
  • Genetics(clinical)

Fingerprint Dive into the research topics of 'A whole genome methylation analysis of systemic lupus erythematosus: Hypomethylation of the IL10 and IL1R2 promoters is associated with disease activity'. Together they form a unique fingerprint.

  • Cite this

    Lin, S. Y., Hsieh, S. C., Lin, Y. C., Lee, C. N., Tsai, M. H., Lai, L. C., Chuang, E. Y., Chen, P. C., Hung, C. C., Chen, L. Y., Hsieh, W. S., Niu, D. M., Su, Y. N., & Ho, H. N. (2012). A whole genome methylation analysis of systemic lupus erythematosus: Hypomethylation of the IL10 and IL1R2 promoters is associated with disease activity. Genes and Immunity, 13(3), 214-220. https://doi.org/10.1038/gene.2011.74