A viscosity and C-reactive protein sensing technique based on Brownian motion measurements by total-internal-reflective-fluorescent velocimetry

Yu Jui Fan, Yi Hsing Liu, Tzu Heng Wu, Horn Jiunn Sheen, Shiming Lin, Jing Fa Tsai

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

In this study, a two-section clinical sample detection on viscosity and bimolecule sensing technique based on measuring the Brownian velocity of nanobeads has been successfully developed. Bare and antibody-coated fluorescent nanobeads were used to detect various viscous solutions and different concentrations of bio-molecular samples, respectively. The Brownian velocities of the nanobeads became slower when the sizes of antibody-coated nanobeads increased during the antibody-antigen interaction process or when the bare nanobeads were seeded in a higher viscosity solution. In order to obtain the characteristics of the Brownian motion of nanobeads in the static liquid, total internal reflection fluorescent velocimetry (TIRFV) was employed. This sensing technique should be useful in clinical diagnosis, bio-molecule judgment, and the study of fluid characteristics.

Original languageEnglish
Title of host publicationIEEE Sensors 2010 Conference, SENSORS 2010
Pages395-400
Number of pages6
DOIs
Publication statusPublished - 2010
Externally publishedYes
Event9th IEEE Sensors Conference 2010, SENSORS 2010 - Waikoloa, HI, United States
Duration: Nov 1 2010Nov 4 2010

Conference

Conference9th IEEE Sensors Conference 2010, SENSORS 2010
CountryUnited States
CityWaikoloa, HI
Period11/1/1011/4/10

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ASJC Scopus subject areas

  • Electrical and Electronic Engineering

Cite this

Fan, Y. J., Liu, Y. H., Wu, T. H., Sheen, H. J., Lin, S., & Tsai, J. F. (2010). A viscosity and C-reactive protein sensing technique based on Brownian motion measurements by total-internal-reflective-fluorescent velocimetry. In IEEE Sensors 2010 Conference, SENSORS 2010 (pp. 395-400). [5690216] https://doi.org/10.1109/ICSENS.2010.5690216