A viral kinase mimics S6 kinase to enhance cell proliferation

Aadra Prashant Bhatt, Jason P. Wong, Marc S. Weinberg, Kurtis M. Host, Louise C. Giffin, Joshua Buijnink, Evert Van Dijk, Yoshihiro Izumiya, Hsing Jien Kung, Brenda R.S. Temple, Blossom Damania

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Viruses depend upon the host cell for manufacturing components of progeny virions. To mitigate the inextricable dependence on host cell protein synthesis, viruses can modulate protein synthesis through a variety of mechanisms. We report that the viral protein kinase (vPK) encoded by open reading frame 36 (ORF36) of Kaposi's sarcoma-associated herpesvirus (KSHV) enhances protein synthesis by mimicking the function of the cellular protein S6 kinase (S6KB1). Similar to S6KB1, vPK phosphorylates the ribosomal S6 protein and up-regulates global protein synthesis. vPK also augments cellular proliferation and anchorage-independent growth. Furthermore, we report that both vPK and S6KB1 phosphorylate the enzyme 6-phosphofructo-2-kinase/fructose-2, 6-bisphosphatase 2 (PFKFB2) and that both kinases promote endothelial capillary tubule formation.

Original languageEnglish
Pages (from-to)7876-7881
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume113
Issue number28
DOIs
Publication statusPublished - Jul 12 2016
Externally publishedYes

Fingerprint

Ribosomal Protein S6 Kinases
Protein Kinases
Viral Proteins
Phosphotransferases
Cell Proliferation
Proteins
Phosphofructokinase-2
Ribosomal Protein S6
Viruses
Human Herpesvirus 8
Cellular Structures
Virion
Open Reading Frames
Up-Regulation
Enzymes
Growth

Keywords

  • Cell signaling
  • KSHV
  • ORF36
  • S6K
  • Viral protein kinase

ASJC Scopus subject areas

  • General

Cite this

Bhatt, A. P., Wong, J. P., Weinberg, M. S., Host, K. M., Giffin, L. C., Buijnink, J., ... Damania, B. (2016). A viral kinase mimics S6 kinase to enhance cell proliferation. Proceedings of the National Academy of Sciences of the United States of America, 113(28), 7876-7881. https://doi.org/10.1073/pnas.1600587113

A viral kinase mimics S6 kinase to enhance cell proliferation. / Bhatt, Aadra Prashant; Wong, Jason P.; Weinberg, Marc S.; Host, Kurtis M.; Giffin, Louise C.; Buijnink, Joshua; Van Dijk, Evert; Izumiya, Yoshihiro; Kung, Hsing Jien; Temple, Brenda R.S.; Damania, Blossom.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 113, No. 28, 12.07.2016, p. 7876-7881.

Research output: Contribution to journalArticle

Bhatt, AP, Wong, JP, Weinberg, MS, Host, KM, Giffin, LC, Buijnink, J, Van Dijk, E, Izumiya, Y, Kung, HJ, Temple, BRS & Damania, B 2016, 'A viral kinase mimics S6 kinase to enhance cell proliferation', Proceedings of the National Academy of Sciences of the United States of America, vol. 113, no. 28, pp. 7876-7881. https://doi.org/10.1073/pnas.1600587113
Bhatt, Aadra Prashant ; Wong, Jason P. ; Weinberg, Marc S. ; Host, Kurtis M. ; Giffin, Louise C. ; Buijnink, Joshua ; Van Dijk, Evert ; Izumiya, Yoshihiro ; Kung, Hsing Jien ; Temple, Brenda R.S. ; Damania, Blossom. / A viral kinase mimics S6 kinase to enhance cell proliferation. In: Proceedings of the National Academy of Sciences of the United States of America. 2016 ; Vol. 113, No. 28. pp. 7876-7881.
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