A tyrosine kinase profile of prostate carcinoma

Dan Robinson, Feng He, Tom Pretlow, Hsing Jien Kung

Research output: Contribution to journalArticle

192 Citations (Scopus)

Abstract

Tyrosine kinases play central roles in the growth and differentiation of normal and tumor cells. In this study, we have analyzed the general tyrosine kinase expression profile of a prostate carcinoma (PCA) xenograft, CWR2.2. We describe here an improved reverse transcriptase-PCR approach that permits identification of nearly 40 different kinases in a single screening; several of these kinases are newly cloned kinases and some are novel. According to this, there are 11 receptor kinases, 9 nonreceptor kinases, and at least 7 dual kinases expressed in the xenograft tissue. The receptor kinases include erbB2, erbB3, Ret, platelet-derived growth factor receptor, sky, nyk, eph, htk, sek (eph), ddr, and tkt. The nonreceptor kinases are lck, yes, abl, arg, jak1, tyk2, and etk/bmx. Most of the dual kinases are in the mitogen- activating protein (MAP) kinase-kinase (MKK) family, which includes MKK3, MKK4, MEK5, and a novel one. As a complementary approach, we also analyzed by specific reverse transcriptase-PCR primers the expression profile of erbB/epidermal growth factor receptor family receptors in a variety of PCA specimens, cell lines, and benign prostatic hyperplasia. We found that erbB1, -2, and -3 are often coexpressed in prostate tissues, but nut in erbB4. The information established here should provide a base line to study the possible growth and oncogenic signals of PCA.

Original languageEnglish
Pages (from-to)5958-5962
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume93
Issue number12
DOIs
Publication statusPublished - Jun 11 1996
Externally publishedYes

Keywords

  • erbB kinase
  • human xenograft
  • oncogene
  • reverse transcriptase-PCR
  • signal transduction

ASJC Scopus subject areas

  • Genetics
  • General

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