A three–MicroRNA signature as a potential biomarker for the early detection of oral cancer

Yi An Chang, Shun Long Weng, Shun Fa Yang, Chih Hung Chou, Wei Chih Huang, Siang Jyun Tu, Tzu Hao Chang, Chien Ning Huang, Yuh Jyh Jong, Hsien Da Huang

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Oral squamous cell carcinoma (OSCC) is often diagnosed at a late stage and may be malignantly transformed from oral leukoplakia (OL). This study aimed to identify potential plasma microRNAs (miRNAs) for the early detection of oral cancer. Plasma from normal, OL, and OSCC patients were evaluated. Small RNA sequencing was used to screen the differently expressed miRNAs among the groups. Next, these miRNAs were validated with individual samples by quantitative real-time polymerase chain reaction (qRT-PCR) assays in the training phase (n = 72) and validation phase (n = 178). The possible physiological roles of the identified miRNAs were further investigated using bioinformatics analysis. Three miRNAs (miR-222-3p, miR-150-5p, and miR-423-5p) were identified as differentially expressed among groups; miR-222-3p and miR-423-5p negatively correlated with T stage, lymph node metastasis status, and clinical stage. A high diagnostic accuracy (Area under curve = 0.88) was demonstrated for discriminating OL from OSCC. Bioinformatics analysis reveals that miR-423-5p and miR-222-3p are significantly over-expressed in oral cancer tissues and involved in various cancer pathways. The three-plasma miRNA panel may be useful to monitor malignant progression from OL to OSCC and as potential biomarkers for early detection of oral cancer.

Original languageEnglish
Article number758
JournalInternational Journal of Molecular Sciences
Volume19
Issue number3
DOIs
Publication statusPublished - Mar 7 2018

Fingerprint

biomarkers
Mouth Neoplasms
Biomarkers
MicroRNAs
Early Detection of Cancer
Oral Leukoplakia
cancer
signatures
Squamous Cell Carcinoma
Bioinformatics
Plasmas
Computational Biology
Polymerase chain reaction
RNA
RNA Sequence Analysis
Assays
Tissue
polymerase chain reaction
Area Under Curve
sequencing

Keywords

  • Biomarker
  • Early diagnosis
  • Leukoplakia
  • MiRNA
  • Oral cancer

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Chang, Y. A., Weng, S. L., Yang, S. F., Chou, C. H., Huang, W. C., Tu, S. J., ... Huang, H. D. (2018). A three–MicroRNA signature as a potential biomarker for the early detection of oral cancer. International Journal of Molecular Sciences, 19(3), [758]. https://doi.org/10.3390/ijms19030758

A three–MicroRNA signature as a potential biomarker for the early detection of oral cancer. / Chang, Yi An; Weng, Shun Long; Yang, Shun Fa; Chou, Chih Hung; Huang, Wei Chih; Tu, Siang Jyun; Chang, Tzu Hao; Huang, Chien Ning; Jong, Yuh Jyh; Huang, Hsien Da.

In: International Journal of Molecular Sciences, Vol. 19, No. 3, 758, 07.03.2018.

Research output: Contribution to journalArticle

Chang, YA, Weng, SL, Yang, SF, Chou, CH, Huang, WC, Tu, SJ, Chang, TH, Huang, CN, Jong, YJ & Huang, HD 2018, 'A three–MicroRNA signature as a potential biomarker for the early detection of oral cancer', International Journal of Molecular Sciences, vol. 19, no. 3, 758. https://doi.org/10.3390/ijms19030758
Chang, Yi An ; Weng, Shun Long ; Yang, Shun Fa ; Chou, Chih Hung ; Huang, Wei Chih ; Tu, Siang Jyun ; Chang, Tzu Hao ; Huang, Chien Ning ; Jong, Yuh Jyh ; Huang, Hsien Da. / A three–MicroRNA signature as a potential biomarker for the early detection of oral cancer. In: International Journal of Molecular Sciences. 2018 ; Vol. 19, No. 3.
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