A tailored biomimetic hydrogel as potential bioink to print a cell scaffold for tissue engineering applications: Printability and cell viability evaluation

Shyuan Yow Chen, Yung Chieh Cho, Tzu Sen Yang, Keng Liang Ou, Wen Chien Lan, Bai Hung Huang, Takashi Saito, Chi Hsun Tsai, Bou Yue Peng, Yen Chun Chuo, Hung Yang Lin, Hsiao Wei Chao, Christopher J. Walinski, Muhammad Ruslin

Research output: Contribution to journalArticlepeer-review

Abstract

The present study established a maximum standard for printing quality and developed a preliminary ideal index to print three-dimensional (3D) construct using the Gly-Arg-Gly-Asp (GRGD) peptide modified Pluronic-F127 hydrogel (hereafter defined as 3DG bioformer (3BE)) as bioink. In addition, the biocompatibility of 3BE for 3D printing applications was carefully investigated. For biocompatibility study and ideal printing parameter, we used the formulation of 3BE in three different concentrations (3BE-1: 25%, 3BE-2: 30%, and 3BE-3: 35%). The 3BE hydrogels were printed layer by layer as a cube-like construct with all diameters of the needle head under the same feed (100 mm/s). The printing parameters were determined using combinations of 3BE-1, 3BE-2, and 3BE-3 with three different standard needle sizes (F 0.13 mm, Φ 0.33 mm, and Φ 0.9 mm). The printed constructs were photographed and observed using optical microscopy. The cell viability and proliferation were evaluated using Live/Dead assay and immunofluorescence staining. Results showed that a stable of printed line and construct could be generated from the 3BE-3 combinations. Cytotoxicity assay indicated that the 3BE hydrogels possessed well biocompatibility. Bioprinting results also demonstrated that significant cell proliferation in the 3BE-3 combinations was found within three days of printing. Therefore, the study discovered the potential printing parameters of 3BE as bioink to print a stable construct that may also have high biocompatibility for cell encapsulation. This finding could serve as valuable information in creating a functional scaffold for tissue engineering applications.

Original languageEnglish
Article number829
Pages (from-to)1-12
Number of pages12
JournalApplied Sciences (Switzerland)
Volume11
Issue number2
DOIs
Publication statusPublished - Jan 2021

Keywords

  • Bioprinting
  • Gly-arg-gly-asp peptide
  • Hydrogel
  • Pluronic F127
  • Printing parameters
  • Scaffold

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation
  • Engineering(all)
  • Process Chemistry and Technology
  • Computer Science Applications
  • Fluid Flow and Transfer Processes

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