A soft coral natural product, 11-episinulariolide acetate, inhibits gene expression of cyclooxygenase-2 and interleukin-8 through attenuation of calcium signaling

Wen Li Hsu, Siou Jin Chiu, Yao Ting Tsai, Che Mai Chang, Jaw Yan Wang, Eric Terry Wang, Ming Feng Hou, Chiung Yao Huang, Jyh Horng Sheu, Wei Chiao Chang

Research output: Contribution to journalArticlepeer-review

9 Citations (Scopus)

Abstract

Epidermal growth factor receptor (EGFR) is overexpressed in many types of cancer cells. EGFR-mediated signaling involves inflammatory gene expression including cyclooxygenase (COX)-2 and interleukin (IL)-8, and is associated with cancer pathogenesis. In a search of phytochemicals with anti-inflammatory activity, the COX-2 and IL-8 inhibitory activities of some marine compounds were examined. After screening these compounds 11-episinulariolide acetate (1) from soft coral exhibited the most potent activity. Reverse-transcription PCR; western blotting; ELISA and luciferase assays were used to test the effect of compound 1 on EGF-stimulated expressions of COX-2 and IL-8 in A431 human epidermoid carcinoma cells. After exposure to 10 μM of compound 1, expression levels of COX-2 and IL-8 were reduced. In addition; intracellular Ca 2+ increase and Ca2+-dependent transcription factor activation were blocked by compound 1. Thus, compound 1 can potentially serve as a lead compound for targeting Ca2+ signaling-dependent inflammatory diseases.

Original languageEnglish
Pages (from-to)7023-7034
Number of pages12
JournalMolecules
Volume18
Issue number6
DOIs
Publication statusPublished - Jun 2013

Keywords

  • Calcium
  • COX-2
  • EGF
  • IL-8
  • Marine compound

ASJC Scopus subject areas

  • Organic Chemistry
  • Medicine(all)

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