A Smartphone-based Diffusometric Immunoassay for Detecting C-Reactive Protein

Chih Shen Chuang, Chih Zong Deng, Yi Fan Fang, Hong Ren Jiang, Pao Wei Tseng, Horn Jiunn Sheen, Yu Jui Fan

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Abstract

In this study, we developed a portable smartphone-based diffusometry for analyzing the C-reactive protein (CRP) concentration. An optimized fluorescence microscopic add-on system for a smartphone was used to image the 300 nm fluorescent beads. Sequential nanobead images were recorded for a period and the image data were used for fluorescence correlation spectrometric (FCS) analysis. Through the analysis, the nanobeads’ diffusion coefficient was obtained. Further, the diffusion coefficients of the anti-CRP-coated nanobeads, which were suspended in the samples with various CRP concentrations, were estimated using smartphone-based diffusometry. After 10 min of reaction, the anti-CRP-coated nanobeads in a higher CRP concentration solution led to a lower diffusion coefficient. Based on the experiments, a linear sensing range of 1~8 µg/mL was found.

Original languageEnglish
Article number17131
JournalScientific Reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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Cite this

Chuang, C. S., Deng, C. Z., Fang, Y. F., Jiang, H. R., Tseng, P. W., Sheen, H. J., & Fan, Y. J. (2019). A Smartphone-based Diffusometric Immunoassay for Detecting C-Reactive Protein. Scientific Reports, 9(1), [17131]. https://doi.org/10.1038/s41598-019-52285-4