A simplified technique for embryo biopsy: Use of the same micropipette for zona drilling and blastomere aspiration

Shee Uan Chen, Hong Nerng Ho, Hsin Fu Chen, Kuang Han Chao, Su Cheng Huang, Tzu Yao Lee, Yu Shih Yang

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Purpose: Using different micropipettes for zona drilling and blastomere aspiration for embryo biopsy is prevalent at centers of preimplantation genetic diagnosis. The purpose of our study was to simplify the technique by using only one micropipette. Methods: In this animal model, ICR mouse embryos at the four-cell stage (n = 446) were randomly allocated into two groups: a biopsied group (n = 224)for blastomere aspiration and a control group (n = 222) without micromanipulation. We used a drilling/biopsy micropipette to drill a hole in the zona by expulsion of acidified Tyrode's solution and to aspirate the blastomere by gentle suction with the same micropipette and pull it out of the zona. One blastomere was biopsied from each embryo. Results: In all, 222 (99.1%) intact blastomeres were successfully biopsied from 224 embryos. Only two blastomeres were damaged during aspiration. The capacity for blastocyst development (92.4 vs 93.7%) was not different between the two groups, but the percentages of embryos hatching (51.8 vs 18.0%) and hatched (29.9 vs 8.1%) were significantly higher in the biopsied group than in the control group. Conclusions: This simplified technique of embryo biopsy is safe and highly efficient for obtaining blastomeres for preimplantation genetic diagnosis and may also facilitate hatching of the blastocysts.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalJournal of Assisted Reproduction and Genetics
Volume14
Issue number3
DOIs
Publication statusPublished - Jan 1 1997
Externally publishedYes

Fingerprint

Embryonic and Fetal Development
Blastomeres
Zona Pellucida
Inbred ICR Mouse
Herpes Zoster
Embryonic Development
Embryonic Structures
Biopsy
Pregnancy
Preimplantation Diagnosis
Blastocyst
Micromanipulation
Control Groups
Suction
Needle Biopsy
Animal Models

Keywords

  • assisted hatching
  • blastomere biopsy
  • zona drilling

ASJC Scopus subject areas

  • Reproductive Medicine
  • Genetics
  • Obstetrics and Gynaecology
  • Developmental Biology
  • Genetics(clinical)

Cite this

A simplified technique for embryo biopsy : Use of the same micropipette for zona drilling and blastomere aspiration. / Chen, Shee Uan; Ho, Hong Nerng; Chen, Hsin Fu; Chao, Kuang Han; Huang, Su Cheng; Lee, Tzu Yao; Yang, Yu Shih.

In: Journal of Assisted Reproduction and Genetics, Vol. 14, No. 3, 01.01.1997, p. 157-161.

Research output: Contribution to journalArticle

Chen, Shee Uan ; Ho, Hong Nerng ; Chen, Hsin Fu ; Chao, Kuang Han ; Huang, Su Cheng ; Lee, Tzu Yao ; Yang, Yu Shih. / A simplified technique for embryo biopsy : Use of the same micropipette for zona drilling and blastomere aspiration. In: Journal of Assisted Reproduction and Genetics. 1997 ; Vol. 14, No. 3. pp. 157-161.
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