A simple, rapid, high-resolution chromosome technic for lymphocytes

Y. S. Kao, J. Whang-Peng, E. Lee

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Phytohemagglutinin (PHA) stimulated lymphocytes were exposed to a hypotonic solution consisting of equal parts of 0.075 M 2-Mercaptoethanol and 0.075 M KCl, followed by addition of colcemid. The cells were fixed and washed in the usual manner. The slides then were subjected to trypsin-Giemsa banding. This method yields a high percentage of mitotic figures in prophase and prometaphase, ideal for high-resolution chromosome analysis.

Original languageEnglish
Pages (from-to)481-483
Number of pages3
JournalAmerican Journal of Clinical Pathology
Volume79
Issue number4
DOIs
Publication statusPublished - Jan 1 1983
Externally publishedYes

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Hypotonic Solutions
Prometaphase
Demecolcine
Prophase
Mercaptoethanol
Phytohemagglutinins
Trypsin
Chromosomes
Lymphocytes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

A simple, rapid, high-resolution chromosome technic for lymphocytes. / Kao, Y. S.; Whang-Peng, J.; Lee, E.

In: American Journal of Clinical Pathology, Vol. 79, No. 4, 01.01.1983, p. 481-483.

Research output: Contribution to journalArticle

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