A simple plant gene delivery system using mesoporous silica nanoparticles as carriers

Feng Peng Chang, Lin Yun Kuang, Chia An Huang, Wann Neng Jane, Yann Hung, Yue Ie C. Hsing, Chung Yuan Mou

Research output: Contribution to journalArticlepeer-review

87 Citations (Scopus)

Abstract

A facile DNA delivery method would greatly facilitate studies of plant functional genomics. However, plant cell walls limit the utilization of nanoparticles on plant research. Here, we employed functionalized mesoporous silica nanoparticles (MSNs) to develop a MSN-mediated plant transient gene expression system. In this system, MSNs served as carriers to deliver foreign DNA into intact Arabidopsis thaliana roots without the aid of mechanical force. Gene expression was detected in the epidermal layer and in the more inner cortical and endodermal root tissues by both fluorescence and antibody labeling. This is a novel alternative to the conventional gene-gun or ultrasonic methods. In addition, the parameters that affect the MSN uptake and the mechanism and subcellular distribution of particles were also analyzed. The present study may provide valuable information on the manipulation of functional nanoparticles in plants and have significant impact on plant biotechnology.

Original languageEnglish
Pages (from-to)5279-5287
Number of pages9
JournalJournal of Materials Chemistry B
Volume1
Issue number39
DOIs
Publication statusPublished - Oct 21 2013
Externally publishedYes

ASJC Scopus subject areas

  • Chemistry(all)
  • Biomedical Engineering
  • Materials Science(all)

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