A seminal vesicle autoantigen of mouse is able to suppress sperm capacitation-related events stimulated by serum albumin

Y. H. Huang, S. T. Chu, Y. H. Chen

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

We studied the effect of a mouse seminal vesicle autoantigen (SVA) on BSA-stimulated functions of mouse sperm. Uncapacitated, capacitated, and acrosome-reacted stages of sperm were morphologically scored, and the cellular zinc content was examined cytologically in a modified Tyrode solution at 37°C for 80 min. More than 85% of control cells remained uncapacitated. Addition of 0.3% SVA to the cell incubation did not affect the cell status. Approximately 65% of cells were capacitated in the incubation medium containing 0.3% BSA. Only 30% of the cells became capacitated after incubation with 0.3% BSA and 0.3% SVA together. The decapacitation effect by 0.3% SVA could be subdued by more than 3% BSA in the cell incubation. Whereas BSA did, SVA did not cause removal of Zn2+ from sperm, but SVA could suppress the BSA effect. The tyrosine phosphorylated proteins in sperm were detected after incubation in a modified HEPES medium containing 0.3% BSA and/or 0.3% SVA at 37°C for 90 min. Whereas BSA enhanced greatly, SVA did not cause phosphorylation of proteins in the range of M(r) 40 000-120 000. The BSA-stimulated protein tyrosine phosphorylation could be suppressed by SVA in the cell incubation.

Original languageEnglish
Pages (from-to)1562-1566
Number of pages5
JournalBiology of Reproduction
Volume63
Issue number5
Publication statusPublished - 2000
Externally publishedYes

Keywords

  • Seminal vesicles
  • Sperm motility and transport

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Embryology

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