A screening platform for compounds with potential immuno-regulatory activities using human cord blood mononuclear cells

Chia Jung Chen, Che Tsai Chia, Jung Feng Hsieh, Chichen Michael Chien, Tzu H. Wu, Shui Tein Chen

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

A systematic and combinatorial approach was adopted using human umbilical cord blood mononuclear cells (hUCB-MNCs) to screen for potential immuno-regulatory compounds. The hUCB-MNCs contain several types of immunogenic cells, which are a suitable material to mimic the in vivo immuno-response after drug treatment. hUCB-MNCs were treated with various natural products such as quercetin, astaxanthin, caffeic acid, bilobalide, eugenol, rutin and γ-dodecalactone (γ-DDL). Phenotypic expression analysis revealed that the subpopulation of CD3+ T cells, CD56+ NK cells and CD1a+ dendritic cells apparently increased after being treated with γ-DDL for 6 days. The expression of CD56 reached a maximum at 72 h with a dose-dependent relationship. The NK cells activation marker (CD69) also elevated following γ-DDL treatment. These results demonstrated that the γ-DDL has immuno-regulatory effects to enhance cord blood NK cells population and bioactivities. Such a high-throughput methodology using hUCB-MNCs may be an effective platform for systematically screening potential immuno-regulatory compounds.

Original languageEnglish
Pages (from-to)777-784
Number of pages8
JournalCombinatorial Chemistry and High Throughput Screening
Volume9
Issue number10
DOIs
Publication statusPublished - Dec 2006

Keywords

  • γ-dodecalactone
  • Flow cytometry
  • High-throughput screening
  • Human umbilical cord blood mononuclear cells
  • Immuno-regulatory activities
  • Natural products

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Chemistry (miscellaneous)
  • Pharmacology

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