A Review of the Major Penaeid Shrimp EST Studies and the Construction of a Shrimp Transcriptome Database Based on the ESTs from Four Penaeid Shrimp

Jiann Horng Leu, Shu Hwa Chen, Yu Bin Wang, Yen Chen Chen, Sheng Yao Su, Chung Yen Lin, Jan Ming Ho, Chu Fang Lo

Research output: Contribution to journalReview article

38 Citations (Scopus)

Abstract

By economic value, shrimp is currently the most important seafood commodity worldwide, and these animals are often the subject of scientific research in shrimp farming countries. High throughput methods, such as expressed sequence tags (ESTs), were originally developed to study human genomics, but they are now available for studying other important organisms, including shrimp. ESTs are short sequences generated by sequencing randomly selected cDNA clones from a cDNA library. This is currently the most efficient and powerful method for providing transcriptomic data for organisms with an uncharacterized genome. This review will summarize the sixteen major shrimp EST studies that have been conducted to date. In addition, we analyzed the EST data downloaded from NCBI dbEST for the four major penaeid shrimp species and constructed a database to host all of these EST data as well as our own analysis results. This database provides the shrimp aquaculture research community with an outline of the shrimp transcriptome as well as a tool for shrimp gene identification.

Original languageEnglish
Pages (from-to)608-621
Number of pages14
JournalMarine Biotechnology
Volume13
Issue number4
DOIs
Publication statusPublished - Aug 1 2011
Externally publishedYes

Fingerprint

Penaeidae
Expressed Sequence Tags
expressed sequence tags
Transcriptome
transcriptome
shrimp
Databases
seafood
commodity
aquaculture
clone
genomics
genome
Seafood
Aquaculture
Genomics
shrimp culture
Agriculture
Gene Library
Research

Keywords

  • cDNA libraries
  • EST
  • Gene expression
  • Penaeid shrimp
  • Transcriptome

ASJC Scopus subject areas

  • Biotechnology
  • Aquatic Science

Cite this

A Review of the Major Penaeid Shrimp EST Studies and the Construction of a Shrimp Transcriptome Database Based on the ESTs from Four Penaeid Shrimp. / Leu, Jiann Horng; Chen, Shu Hwa; Wang, Yu Bin; Chen, Yen Chen; Su, Sheng Yao; Lin, Chung Yen; Ho, Jan Ming; Lo, Chu Fang.

In: Marine Biotechnology, Vol. 13, No. 4, 01.08.2011, p. 608-621.

Research output: Contribution to journalReview article

Leu, Jiann Horng ; Chen, Shu Hwa ; Wang, Yu Bin ; Chen, Yen Chen ; Su, Sheng Yao ; Lin, Chung Yen ; Ho, Jan Ming ; Lo, Chu Fang. / A Review of the Major Penaeid Shrimp EST Studies and the Construction of a Shrimp Transcriptome Database Based on the ESTs from Four Penaeid Shrimp. In: Marine Biotechnology. 2011 ; Vol. 13, No. 4. pp. 608-621.
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