A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis

Chien Han Kao, Chiu Min Cheng, Kuo Hsiang Chuang, Chih Hung Chuang, Shey Cherng Tzou, Ta Chun Cheng, Yuan Chin Hsieh, Kuang Wen Liao, Yun Ming Wang, Long Sen Chang, Steve R. Roffler, Fang Ming Chen, Tian Lu Cheng

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We designed a protein ladder (hereafter referred to as "Mega- tag") that contains 14 of the most commonly used epitope tags fused to molecular weight markers. The Mega-tag ladder can be simultaneously visualized when anti-tag antibodies are used to detect epitope-tagged recombinant proteins in Western blots. The logarithm of molecular weights and relative mobility of the Mega-tag protein ladder are highly correlated (R2= 0.997 ± 0.00232), indicating that the dye-free Mega-tag protein ladder is accurate. It can also serve as a positive control for anti-epitope tag immunoblots. The Mega-tag protein ladder should provide a convenient and precise tool for Western blot analysis.

Original languageEnglish
Pages (from-to)1-3
Number of pages3
JournalAnalytical Biochemistry
Volume431
Issue number1
DOIs
Publication statusPublished - Dec 1 2012

Fingerprint

Ladders
Western Blotting
Epitopes
Proteins
Molecular Weight
Molecular weight
Recombinant Proteins
Anti-Idiotypic Antibodies
Coloring Agents
Antibodies

Keywords

  • 14-Epitope tag protein ladder
  • Anti-epitope tag antibodies
  • Epitope tags
  • Protein molecular estimation
  • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
  • Western blot analysis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology
  • Cell Biology

Cite this

Kao, C. H., Cheng, C. M., Chuang, K. H., Chuang, C. H., Tzou, S. C., Cheng, T. C., ... Cheng, T. L. (2012). A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis. Analytical Biochemistry, 431(1), 1-3. https://doi.org/10.1016/j.ab.2012.08.001

A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis. / Kao, Chien Han; Cheng, Chiu Min; Chuang, Kuo Hsiang; Chuang, Chih Hung; Tzou, Shey Cherng; Cheng, Ta Chun; Hsieh, Yuan Chin; Liao, Kuang Wen; Wang, Yun Ming; Chang, Long Sen; Roffler, Steve R.; Chen, Fang Ming; Cheng, Tian Lu.

In: Analytical Biochemistry, Vol. 431, No. 1, 01.12.2012, p. 1-3.

Research output: Contribution to journalArticle

Kao, CH, Cheng, CM, Chuang, KH, Chuang, CH, Tzou, SC, Cheng, TC, Hsieh, YC, Liao, KW, Wang, YM, Chang, LS, Roffler, SR, Chen, FM & Cheng, TL 2012, 'A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis', Analytical Biochemistry, vol. 431, no. 1, pp. 1-3. https://doi.org/10.1016/j.ab.2012.08.001
Kao, Chien Han ; Cheng, Chiu Min ; Chuang, Kuo Hsiang ; Chuang, Chih Hung ; Tzou, Shey Cherng ; Cheng, Ta Chun ; Hsieh, Yuan Chin ; Liao, Kuang Wen ; Wang, Yun Ming ; Chang, Long Sen ; Roffler, Steve R. ; Chen, Fang Ming ; Cheng, Tian Lu. / A regularly spaced and self-revealing protein ladder for anti-tag Western blot analysis. In: Analytical Biochemistry. 2012 ; Vol. 431, No. 1. pp. 1-3.
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