A recA null mutation may be generated in Streptomyces coelicolor

Tzu-Wen Huang, Hsiuh-Wei Chen

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The recombinase RecA plays a crucial role in homologous recombination and the SOS response in bacteria. Although recA mutants usually are defective in homologous recombination and grow poorly, they nevertheless can be isolated in almost all bacteria. Previously, considerable difficulties were experienced by several laboratories in generating recA null mutations in Streptomyces, and the only recA null mutants isolated (from Streptomyces lividans) appeared to be accompanied by a suppressing mutation. Using gene replacement mediated by Escherichia coli-Streptomyces conjugation, we generated recA null mutations in a series of Streptomyces coelicolor A3 (2) strains. These recA mutants were very sensitive to mitomycin C but only moderately sensitive to UV irradiation, and the UV survival curves showed wide shoulders, reflecting the presence of a recA-independent repair pathway. The mutants segregated minute colonies with low viability during growth and produced more anucleate spores than the wild type. Some crosses between pairs of recA null mutants generated no detectable recombinants, showing for the first time that conjugal recombination in S. coelicolor is recA mediated, but other mutants retained the ability to undergo recombination. The nature of this novel recombination activity is unknown. Copyright © 2006, American Society for Microbiology. All Rights Reserved.
Original languageEnglish
Pages (from-to)6771-6779
Number of pages9
JournalJournal of Bacteriology
Volume188
Issue number19
DOIs
Publication statusPublished - 2006
Externally publishedYes

Keywords

  • gene product
  • mitomycin C
  • mutant protein
  • RecA protein
  • recombinase
  • article
  • bacterial gene
  • bacterial genetics
  • bacterial growth
  • bacterial mutation
  • bacterial spore
  • bacterial strain
  • bacterial survival
  • bacterium colony
  • bacterium conjugation
  • bacterium isolation
  • bacterium mutant
  • cell viability
  • controlled study
  • DNA repair
  • drug sensitivity
  • Escherichia coli
  • gene mutation
  • homologous recombination
  • nonhuman
  • null allele
  • priority journal
  • radiosensitivity
  • RecA gene
  • Streptomyces coelicolor
  • Streptomyces lividans
  • ultraviolet radiation
  • Anti-Bacterial Agents
  • Conjugation, Genetic
  • Gene Deletion
  • Mitomycin
  • Rec A Recombinases
  • Recombination, Genetic
  • Ultraviolet Rays
  • Streptomyces

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