A real-time impedance-sensing chip for the detection of emulsion phase separation

Yung Sheng Lin, Wei Lung Chou, Chih Hui Yang, Keng Shiang Huang, Eng Chi Wang, Cheng You Chen, Yu Hsin Lin, Haw Ming Huang

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

This paper describes a novel real-time impedance chip for the detection of squalene-water emulsion phase separation. Each impedance chip contains eight pairs of indium tin oxide microelectrode arrays for detecting eight samples, and six chips can be connected with the switch relay to measure 48 samples in the system simultaneously. The proposed impedance chip has the advantages of needing only a small sample volume (0.5 mL), and provides parallel, continuous, and real-time detection. The effects of the surfactant concentration on the stability of a squalene/water emulsion were studied by means of a visual inspection, a conductance probe, and by impedance chip. Three different concentrations of Tween 20 surfactant (9, 17, and 29 wt%) were employed for the examinations. The results indicated that the phase separation rate was faster in the lower surfactant concentration. However, the emulsion of 29 wt% Tween 20 was fairly stable for more than 2 days since there were no signal changes according to the three detection methods. The reaction time (TR) for completing the measured phase separation process differed for each of the three methods (measuring aqueous phase height, conductance, and impedance, respectively). For the 9 wt% Tween 20, the reaction times were 24 h, 20 min, and 5 min in the tests using visual inspection, conductance probe, and impedance chip, respectively. For the 17 wt% Tween 20, the TR was also shorter when using the impedance chip method compared to the other two methods. Therefore the proposed impedance chip has a quick reaction response and provides an alternative and effective method to detect emulsion stability.

Original languageEnglish
Pages (from-to)1743-1748
Number of pages6
JournalElectrophoresis
Volume34
Issue number12
DOIs
Publication statusPublished - Jun 2013

Fingerprint

Polysorbates
Emulsions
Electric Impedance
Phase separation
Surface-Active Agents
Squalene
Inspection
Water
Microelectrodes
Switches

Keywords

  • Chip
  • Emulsion
  • Impedance
  • Phase separation
  • Stability

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Biochemistry

Cite this

Lin, Y. S., Chou, W. L., Yang, C. H., Huang, K. S., Wang, E. C., Chen, C. Y., ... Huang, H. M. (2013). A real-time impedance-sensing chip for the detection of emulsion phase separation. Electrophoresis, 34(12), 1743-1748. https://doi.org/10.1002/elps.201200517

A real-time impedance-sensing chip for the detection of emulsion phase separation. / Lin, Yung Sheng; Chou, Wei Lung; Yang, Chih Hui; Huang, Keng Shiang; Wang, Eng Chi; Chen, Cheng You; Lin, Yu Hsin; Huang, Haw Ming.

In: Electrophoresis, Vol. 34, No. 12, 06.2013, p. 1743-1748.

Research output: Contribution to journalArticle

Lin, YS, Chou, WL, Yang, CH, Huang, KS, Wang, EC, Chen, CY, Lin, YH & Huang, HM 2013, 'A real-time impedance-sensing chip for the detection of emulsion phase separation', Electrophoresis, vol. 34, no. 12, pp. 1743-1748. https://doi.org/10.1002/elps.201200517
Lin YS, Chou WL, Yang CH, Huang KS, Wang EC, Chen CY et al. A real-time impedance-sensing chip for the detection of emulsion phase separation. Electrophoresis. 2013 Jun;34(12):1743-1748. https://doi.org/10.1002/elps.201200517
Lin, Yung Sheng ; Chou, Wei Lung ; Yang, Chih Hui ; Huang, Keng Shiang ; Wang, Eng Chi ; Chen, Cheng You ; Lin, Yu Hsin ; Huang, Haw Ming. / A real-time impedance-sensing chip for the detection of emulsion phase separation. In: Electrophoresis. 2013 ; Vol. 34, No. 12. pp. 1743-1748.
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AB - This paper describes a novel real-time impedance chip for the detection of squalene-water emulsion phase separation. Each impedance chip contains eight pairs of indium tin oxide microelectrode arrays for detecting eight samples, and six chips can be connected with the switch relay to measure 48 samples in the system simultaneously. The proposed impedance chip has the advantages of needing only a small sample volume (0.5 mL), and provides parallel, continuous, and real-time detection. The effects of the surfactant concentration on the stability of a squalene/water emulsion were studied by means of a visual inspection, a conductance probe, and by impedance chip. Three different concentrations of Tween 20 surfactant (9, 17, and 29 wt%) were employed for the examinations. The results indicated that the phase separation rate was faster in the lower surfactant concentration. However, the emulsion of 29 wt% Tween 20 was fairly stable for more than 2 days since there were no signal changes according to the three detection methods. The reaction time (TR) for completing the measured phase separation process differed for each of the three methods (measuring aqueous phase height, conductance, and impedance, respectively). For the 9 wt% Tween 20, the reaction times were 24 h, 20 min, and 5 min in the tests using visual inspection, conductance probe, and impedance chip, respectively. For the 17 wt% Tween 20, the TR was also shorter when using the impedance chip method compared to the other two methods. Therefore the proposed impedance chip has a quick reaction response and provides an alternative and effective method to detect emulsion stability.

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