A real time detection of the ovarian tumor associated antigen 1 (OVTA 1) in human serum by quartz crystal microbalance immobilized with anti-OVTA 1 polyclonal chicken IgY antibodies

S. Sadhasivam, Jung Chih Chen, S. Savitha, Feng Huei Lin, Yi Yuan Yang, Chi Hsin Lee

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

In this study, we developed a sensitive quartz crystal microbalance (QCM) sensor which employs the anti-ovarian tumor associated antigen 1 (OVTA 1) IgY polyclonal chicken antibodies on the crystal surface for the detection of OVTA 1 in human serum. The anti-OVTA 1 IgY antibodies were anchored on the thiol-activated sensor surface using 1-ethyl-3[3-dimethylaminopropyl] carbodiimide hydrochloride/N-hydroxysuccinimide (EDC-NHS) coupling. The responses for different concentrations of anti-OVTA 1 antibody were also studied and 40 μg/mL was favorable for homogenous antibody coverage. The QCM sensor was used to monitor both immobilization and the target binding affinity. The anti-OVTA 1 antibody based QCM has allowed the competent detection of OVTA 1 in a high linear range of 0.5-10 μg/mL. The total time needed to complete the detection was as short as 5-6 h. The regeneration studies demonstrated that the proposed sensor was reusable up to 9 cycles with a slight loss in binding affinity. The detection of OVTA 1 in human serum allows a potential exploitation of the anti-OVTA 1 polyclonal antibody based QCM immunoassay for the screening of OVTA 1 antigen.

Original languageEnglish
Pages (from-to)2073-2078
Number of pages6
JournalMaterials Science and Engineering C
Volume32
Issue number7
DOIs
Publication statusPublished - Oct 1 2012

Fingerprint

chickens
Quartz crystal microbalances
antigens
Antigens
quartz crystals
antibodies
Antibodies
microbalances
serums
Tumors
tumors
Sensors
sensors
affinity
Ethyldimethylaminopropyl Carbodiimide
IgY
ovarian tumor associated antigen
immunoassay
hydrochlorides
Sulfhydryl Compounds

Keywords

  • Human serum
  • Immunosensors
  • Ovarian cancer
  • OVTA 1 antigen
  • Polyclonal chicken IgY antibodies
  • QCM

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Mechanical Engineering
  • Mechanics of Materials

Cite this

A real time detection of the ovarian tumor associated antigen 1 (OVTA 1) in human serum by quartz crystal microbalance immobilized with anti-OVTA 1 polyclonal chicken IgY antibodies. / Sadhasivam, S.; Chen, Jung Chih; Savitha, S.; Lin, Feng Huei; Yang, Yi Yuan; Lee, Chi Hsin.

In: Materials Science and Engineering C, Vol. 32, No. 7, 01.10.2012, p. 2073-2078.

Research output: Contribution to journalArticle

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