A quantitative and morphometric study of the transformation of amoeboid microglia into ramified microglia in the developing corpus callosum in rats

C. H. Wu, C. Y. Wen, J. Y. Shieh, E. A. Ling

Research output: Contribution to journalArticle

81 Citations (Scopus)

Abstract

The morphometric and quantitative changes associated with the differentiation of amoeboid microglia into ramified microglial cells in the corpus callosum of rats between 21 d postconception (E21) and 15 d postnatally are described. Using lectin labelling, 5 morphological types of labelled cells (R, SP, KLP, TLP, AP) based on cell body shape, the configuration of their cytoplasmic processes and their staining intensity, were recognised. Round cells (R) and cells with stout processes (SP) were aggregated in the central part of the developing corpus callosum whereas the highly branched labelled cells were distributed at its periphery. When the morphometric data and labelling intensities of labelled cells were analysed with the aid of an image analysis system, the values for cell length, area and perimeter increased as the complexity of branching increased, whereas the lectin-labelling intensity became reduced. Quantitative study showed that the proportion of the different morphological types of lectin-labelled cells peaked at different ages. The sequential peaking of R, SP and highly branched cells with advancing age suggests a similar chronological order of differentiation of R into branched cells. The quantitative study also showed a rapid increase in the density of lectin-labelled cells in the postnatal period between P4 and P8, attributed primarily to the active proliferation of the cell type. The consequent reduction of cell density (after P13) was probably due to cell death, a feature which appeared to increase with development.

Original languageEnglish
Pages (from-to)423-430
Number of pages8
JournalJournal of Anatomy
Volume181
Issue number3
Publication statusPublished - 1992
Externally publishedYes

Fingerprint

Corpus Callosum
neuroglia
Microglia
rats
body shape
Lectins
cells
image analysis
lectins
labelling
animal morphology
Cell Shape
postpartum period
Cell Death
Cell Count
branching
Cell Proliferation
cell death
cell proliferation
Staining and Labeling

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Anatomy

Cite this

A quantitative and morphometric study of the transformation of amoeboid microglia into ramified microglia in the developing corpus callosum in rats. / Wu, C. H.; Wen, C. Y.; Shieh, J. Y.; Ling, E. A.

In: Journal of Anatomy, Vol. 181, No. 3, 1992, p. 423-430.

Research output: Contribution to journalArticle

@article{c80805ff5f4c4978bb97cc3775c294ad,
title = "A quantitative and morphometric study of the transformation of amoeboid microglia into ramified microglia in the developing corpus callosum in rats",
abstract = "The morphometric and quantitative changes associated with the differentiation of amoeboid microglia into ramified microglial cells in the corpus callosum of rats between 21 d postconception (E21) and 15 d postnatally are described. Using lectin labelling, 5 morphological types of labelled cells (R, SP, KLP, TLP, AP) based on cell body shape, the configuration of their cytoplasmic processes and their staining intensity, were recognised. Round cells (R) and cells with stout processes (SP) were aggregated in the central part of the developing corpus callosum whereas the highly branched labelled cells were distributed at its periphery. When the morphometric data and labelling intensities of labelled cells were analysed with the aid of an image analysis system, the values for cell length, area and perimeter increased as the complexity of branching increased, whereas the lectin-labelling intensity became reduced. Quantitative study showed that the proportion of the different morphological types of lectin-labelled cells peaked at different ages. The sequential peaking of R, SP and highly branched cells with advancing age suggests a similar chronological order of differentiation of R into branched cells. The quantitative study also showed a rapid increase in the density of lectin-labelled cells in the postnatal period between P4 and P8, attributed primarily to the active proliferation of the cell type. The consequent reduction of cell density (after P13) was probably due to cell death, a feature which appeared to increase with development.",
author = "Wu, {C. H.} and Wen, {C. Y.} and Shieh, {J. Y.} and Ling, {E. A.}",
year = "1992",
language = "English",
volume = "181",
pages = "423--430",
journal = "Journal of Anatomy",
issn = "0021-8782",
publisher = "Wiley-Blackwell",
number = "3",

}

TY - JOUR

T1 - A quantitative and morphometric study of the transformation of amoeboid microglia into ramified microglia in the developing corpus callosum in rats

AU - Wu, C. H.

AU - Wen, C. Y.

AU - Shieh, J. Y.

AU - Ling, E. A.

PY - 1992

Y1 - 1992

N2 - The morphometric and quantitative changes associated with the differentiation of amoeboid microglia into ramified microglial cells in the corpus callosum of rats between 21 d postconception (E21) and 15 d postnatally are described. Using lectin labelling, 5 morphological types of labelled cells (R, SP, KLP, TLP, AP) based on cell body shape, the configuration of their cytoplasmic processes and their staining intensity, were recognised. Round cells (R) and cells with stout processes (SP) were aggregated in the central part of the developing corpus callosum whereas the highly branched labelled cells were distributed at its periphery. When the morphometric data and labelling intensities of labelled cells were analysed with the aid of an image analysis system, the values for cell length, area and perimeter increased as the complexity of branching increased, whereas the lectin-labelling intensity became reduced. Quantitative study showed that the proportion of the different morphological types of lectin-labelled cells peaked at different ages. The sequential peaking of R, SP and highly branched cells with advancing age suggests a similar chronological order of differentiation of R into branched cells. The quantitative study also showed a rapid increase in the density of lectin-labelled cells in the postnatal period between P4 and P8, attributed primarily to the active proliferation of the cell type. The consequent reduction of cell density (after P13) was probably due to cell death, a feature which appeared to increase with development.

AB - The morphometric and quantitative changes associated with the differentiation of amoeboid microglia into ramified microglial cells in the corpus callosum of rats between 21 d postconception (E21) and 15 d postnatally are described. Using lectin labelling, 5 morphological types of labelled cells (R, SP, KLP, TLP, AP) based on cell body shape, the configuration of their cytoplasmic processes and their staining intensity, were recognised. Round cells (R) and cells with stout processes (SP) were aggregated in the central part of the developing corpus callosum whereas the highly branched labelled cells were distributed at its periphery. When the morphometric data and labelling intensities of labelled cells were analysed with the aid of an image analysis system, the values for cell length, area and perimeter increased as the complexity of branching increased, whereas the lectin-labelling intensity became reduced. Quantitative study showed that the proportion of the different morphological types of lectin-labelled cells peaked at different ages. The sequential peaking of R, SP and highly branched cells with advancing age suggests a similar chronological order of differentiation of R into branched cells. The quantitative study also showed a rapid increase in the density of lectin-labelled cells in the postnatal period between P4 and P8, attributed primarily to the active proliferation of the cell type. The consequent reduction of cell density (after P13) was probably due to cell death, a feature which appeared to increase with development.

UR - http://www.scopus.com/inward/record.url?scp=0027075103&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027075103&partnerID=8YFLogxK

M3 - Article

C2 - 1304580

AN - SCOPUS:0027075103

VL - 181

SP - 423

EP - 430

JO - Journal of Anatomy

JF - Journal of Anatomy

SN - 0021-8782

IS - 3

ER -