A protein factor from Xenopus oocytes with simian virus 40 large tumor antigen-like DNA supercoiling activity

Hui Zhang, C. Bret Jessee, Leroy-Fong Liu

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The ATP-dependent movement of simian virus 40 large tumor antigen on DNA can cause positive supercoiling of template DNA in the presence of bacterial DNA topoisomerase I, which specifically relaxes negative supercoils. With such a supercoiling assay, an activity capable of ATP-dependent, positive supercoiling of DNA in the presence of bacterial DNA topoisomerase I was found in 7S particles prepared from Xenopus laevis oocytes but not in purified transcription factor III A. The positive supercoils accumulated during the reaction are strained, as evidenced by their sensitivity to eukaryotic DNA topoisomerase I. Purification of this activity led to the identification of a DNA-dependent ATPase. Molar excess of transcription factor IIIA or spermidine was found to strongly stimulate the supercoiling reaction of the purified ATPase. These results suggest that this DNA-dependent ATPase can interact with and translocate along closed-circular duplex DNA, producing local alterations in the supercoiled state of the DNA template in a manner analogous to that of simian virus 40 large tumor antigen.

Original languageEnglish
Pages (from-to)9078-9082
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number23
Publication statusPublished - 1990
Externally publishedYes

Fingerprint

Xenopus Proteins
Type I DNA Topoisomerase
Simian virus 40
Neoplasm Antigens
Oocytes
Adenosine Triphosphatases
Bacterial DNA
DNA
Transcription Factor TFIIIA
Adenosine Triphosphate
Superhelical DNA
Circular DNA
Spermidine
Xenopus laevis
Transcription Factors

Keywords

  • ATPase
  • Helicase
  • Topoisomerase

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

@article{2c19781c6deb46a596e412aa246abafb,
title = "A protein factor from Xenopus oocytes with simian virus 40 large tumor antigen-like DNA supercoiling activity",
abstract = "The ATP-dependent movement of simian virus 40 large tumor antigen on DNA can cause positive supercoiling of template DNA in the presence of bacterial DNA topoisomerase I, which specifically relaxes negative supercoils. With such a supercoiling assay, an activity capable of ATP-dependent, positive supercoiling of DNA in the presence of bacterial DNA topoisomerase I was found in 7S particles prepared from Xenopus laevis oocytes but not in purified transcription factor III A. The positive supercoils accumulated during the reaction are strained, as evidenced by their sensitivity to eukaryotic DNA topoisomerase I. Purification of this activity led to the identification of a DNA-dependent ATPase. Molar excess of transcription factor IIIA or spermidine was found to strongly stimulate the supercoiling reaction of the purified ATPase. These results suggest that this DNA-dependent ATPase can interact with and translocate along closed-circular duplex DNA, producing local alterations in the supercoiled state of the DNA template in a manner analogous to that of simian virus 40 large tumor antigen.",
keywords = "ATPase, Helicase, Topoisomerase",
author = "Hui Zhang and {Bret Jessee}, C. and Leroy-Fong Liu",
year = "1990",
language = "English",
volume = "87",
pages = "9078--9082",
journal = "Proceedings of the National Academy of Sciences of the United States of America",
issn = "0027-8424",
publisher = "National Academy of Sciences",
number = "23",

}

TY - JOUR

T1 - A protein factor from Xenopus oocytes with simian virus 40 large tumor antigen-like DNA supercoiling activity

AU - Zhang, Hui

AU - Bret Jessee, C.

AU - Liu, Leroy-Fong

PY - 1990

Y1 - 1990

N2 - The ATP-dependent movement of simian virus 40 large tumor antigen on DNA can cause positive supercoiling of template DNA in the presence of bacterial DNA topoisomerase I, which specifically relaxes negative supercoils. With such a supercoiling assay, an activity capable of ATP-dependent, positive supercoiling of DNA in the presence of bacterial DNA topoisomerase I was found in 7S particles prepared from Xenopus laevis oocytes but not in purified transcription factor III A. The positive supercoils accumulated during the reaction are strained, as evidenced by their sensitivity to eukaryotic DNA topoisomerase I. Purification of this activity led to the identification of a DNA-dependent ATPase. Molar excess of transcription factor IIIA or spermidine was found to strongly stimulate the supercoiling reaction of the purified ATPase. These results suggest that this DNA-dependent ATPase can interact with and translocate along closed-circular duplex DNA, producing local alterations in the supercoiled state of the DNA template in a manner analogous to that of simian virus 40 large tumor antigen.

AB - The ATP-dependent movement of simian virus 40 large tumor antigen on DNA can cause positive supercoiling of template DNA in the presence of bacterial DNA topoisomerase I, which specifically relaxes negative supercoils. With such a supercoiling assay, an activity capable of ATP-dependent, positive supercoiling of DNA in the presence of bacterial DNA topoisomerase I was found in 7S particles prepared from Xenopus laevis oocytes but not in purified transcription factor III A. The positive supercoils accumulated during the reaction are strained, as evidenced by their sensitivity to eukaryotic DNA topoisomerase I. Purification of this activity led to the identification of a DNA-dependent ATPase. Molar excess of transcription factor IIIA or spermidine was found to strongly stimulate the supercoiling reaction of the purified ATPase. These results suggest that this DNA-dependent ATPase can interact with and translocate along closed-circular duplex DNA, producing local alterations in the supercoiled state of the DNA template in a manner analogous to that of simian virus 40 large tumor antigen.

KW - ATPase

KW - Helicase

KW - Topoisomerase

UR - http://www.scopus.com/inward/record.url?scp=0025651703&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0025651703&partnerID=8YFLogxK

M3 - Article

VL - 87

SP - 9078

EP - 9082

JO - Proceedings of the National Academy of Sciences of the United States of America

JF - Proceedings of the National Academy of Sciences of the United States of America

SN - 0027-8424

IS - 23

ER -