A preclinical evaluation of antrodia camphorata alcohol extracts in the treatment of non-small cell lung cancer using non-invasive molecular imaging

Jeng Fong Chiou, Alexander T H Wu, Wei Tin Wang, Tsu Hsiang Kuo, Juri G. Gelovani, I. Hsin Lin, Chih Hsiung Wu, Wen Ta Chiu, Win Ping Deng

Research output: Contribution to journalArticle

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Abstract

This study was carried out to provide a platform for the pre-clinical evaluation of anti-cancer properties of a unique CAM (complementary and alternative medicine) agent, Antrodia camphorata alcohol extract (ACAE), in a mouse model with the advantageous non-invasive in vivo bioluminescence molecular imaging technology. In vitro analyses on the proliferation, migration/invasion, cell cycle and apoptosis were performed on ACAE-treated non-small cell lung cancer cells, H441GL and control CGL1 cells. In vivo, immune-deficient mice were inoculated subcutaneously with H441GL followed by oral gavages of ACAE. The effect of ACAE on tumor progression was monitored by non-invasive bioluminescence imaging. The proliferation and migration/invasion of H441GL cells were inhibited by ACAE in a dose-dependent manner. In addition, ACAE induced cell cycle arrest at G0/G1 phase and apoptosis in H441GL cells as shown by flow cytometric analysis, Annexin-V immunoflourescence and DNA fragmentation. In vivo bioluminescence imaging revealed that tumorigenesis was significantly retarded by oral treatment of ACAE in a dose-dependent fashion. Based on our experimental data, ACAE contains anti-cancer properties and could be considered as a potential CAM agent in future clinical evaluation.

Original languageEnglish
Article number914561
JournalEvidence-based Complementary and Alternative Medicine
Volume2011
DOIs
Publication statusPublished - 2011

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Antrodia
Molecular Imaging
Non-Small Cell Lung Carcinoma
Alcohols
Complementary Therapies
Therapeutics
Apoptosis
Neoplasms
Cell Cycle Resting Phase
Annexin A5
G1 Phase
DNA Fragmentation
Cell Cycle Checkpoints
Cell Cycle
Carcinogenesis
Technology

ASJC Scopus subject areas

  • Complementary and alternative medicine

Cite this

A preclinical evaluation of antrodia camphorata alcohol extracts in the treatment of non-small cell lung cancer using non-invasive molecular imaging. / Chiou, Jeng Fong; Wu, Alexander T H; Wang, Wei Tin; Kuo, Tsu Hsiang; Gelovani, Juri G.; Lin, I. Hsin; Wu, Chih Hsiung; Chiu, Wen Ta; Deng, Win Ping.

In: Evidence-based Complementary and Alternative Medicine, Vol. 2011, 914561, 2011.

Research output: Contribution to journalArticle

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