A potential for granulocyte-colony stimulating factor for use as a prophylactic agent for heatstroke in rats

Ming Chi Yung, Chuan Chih Hsu, Chieh Yi Kang, Chia Li Lin, Shu Ling Chang, Jhi Joung Wang, Mao Tsun Lin, Pei Jarn Chen, Sheng Hsien Chen

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Heatstroke is a form of excessive hyperthermia associated with a systemic inflammatory response that leads to multi-organ dysfunction in which central nervous system disorders predominate. Herein we determined to ascertain whether heat-induced multi-organ dysfunction in rats could be attenuated by granulocyte-colony stimulating factor (G-CSF) preconditioning. Anesthetized rats were divided into 2 major groups and given vehicle solution (isotonic saline, 0.3 ml, subcutaneously) or G-CSF (50-200 μg/kg body weight in 0.3 ml normal saline, subcutaneously) daily and consecutively for 5 days before the start of thermal experiments. They were exposed to an ambient temperature of 43 °C for 68 min to induce heatstroke. G-CSF preconditioning significantly prolonged the survival time in heatstroke rats in a dose-related way (82-98 min vs 127-243 min). The non-preconditioning heatstroke animals showed hyperthermia, arterial hypotension, increased serum levels of systemic inflammatory response molecules, increased hypothalamic apoptotic cell numbers as well as neuronal damage scores, and increased serum levels of renal and hepatic dysfunction indicators. These heatstroke syndromes could be significantly reduced by G-CSF preconditioning. Thus our results revealed a potential for G-CSF used as a prophylactic agent for heatstroke in rats.

Original languageEnglish
Pages (from-to)109-117
Number of pages9
JournalEuropean Journal of Pharmacology
Volume661
Issue number1-3
DOIs
Publication statusPublished - Jul 1 2011
Externally publishedYes

Fingerprint

Heat Stroke
Granulocyte Colony-Stimulating Factor
Fever
Hot Temperature
Isotonic Solutions
Central Nervous System Diseases
Serum
Sodium Chloride
Hypotension
Cell Count
Body Weight
Kidney
Temperature
Liver

Keywords

  • Cytokine
  • Granulocyte-colony stimulating factor
  • Heat stroke
  • Hypothalamus

ASJC Scopus subject areas

  • Pharmacology

Cite this

A potential for granulocyte-colony stimulating factor for use as a prophylactic agent for heatstroke in rats. / Yung, Ming Chi; Hsu, Chuan Chih; Kang, Chieh Yi; Lin, Chia Li; Chang, Shu Ling; Wang, Jhi Joung; Lin, Mao Tsun; Chen, Pei Jarn; Chen, Sheng Hsien.

In: European Journal of Pharmacology, Vol. 661, No. 1-3, 01.07.2011, p. 109-117.

Research output: Contribution to journalArticle

Yung, Ming Chi ; Hsu, Chuan Chih ; Kang, Chieh Yi ; Lin, Chia Li ; Chang, Shu Ling ; Wang, Jhi Joung ; Lin, Mao Tsun ; Chen, Pei Jarn ; Chen, Sheng Hsien. / A potential for granulocyte-colony stimulating factor for use as a prophylactic agent for heatstroke in rats. In: European Journal of Pharmacology. 2011 ; Vol. 661, No. 1-3. pp. 109-117.
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