A potent antioxidant, lycopene, affords neuroprotection against microglia activation and focal cerebral ischemia in rats

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

We investigated the effects of a potent antioxidant, lycopene, on the free radical-scavenging activity as evaluated by the DPPH test and lipid peroxidation in rat brain homogenates as well as nitric oxide (NO) formation in cultured microglia stimulated by lipopolysaccharide. In addition, we also investigated the therapeutic effect of lycopene in attenuating ischemia/reperfusion brain injury induced by middle cerebral artery (MCA) occlusion in rats. Lycopene (1, 2 and 5 μM) exerted increased DPPH decolorization in the DPPH test, and increased inhibition of iron-catalyzed lipid peroxidation (TBARS formation) in rat brain homogenates in concentration-dependent manners. Furthermore, lycopene (5 and 10 μM) significantly inhibited nitrite production by about 31% and 61% in microglia stimulated by LPS, respectively. Rats which received lycopene at a dosage of 4 mg/kg, but not at 2 mg/kg, showed significant infarct size reductions compared with those which received the solvent control (20% Tween 80). In conclusion, we demonstrate a protective effect of lycopene on ischemic brain injury in vivo. Lycopene, through its antioxidative property, mediates at least a portion of free radical-scavenging activity and inhibits microglia activation, resulting in a reduction in infarct volume in ischemia/reperfusion brain injury.

Original languageEnglish
Pages (from-to)351-356
Number of pages6
JournalIn Vivo
Volume18
Issue number3
Publication statusPublished - May 2004

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Microglia
Brain Ischemia
Rats
Antioxidants
Chemical activation
Brain
Brain Injuries
Scavenging
Reperfusion Injury
Lipid Peroxidation
Free Radicals
Lipids
Polysorbates
Middle Cerebral Artery Infarction
Therapeutic Uses
Nitrites
Neuroprotection
lycopene
Lipopolysaccharides
Nitric Oxide

Keywords

  • Antioxidant
  • Ischemia/reperfusion
  • Lycopene
  • MCA occlusion
  • Nitric oxide

ASJC Scopus subject areas

  • Medicine(all)

Cite this

A potent antioxidant, lycopene, affords neuroprotection against microglia activation and focal cerebral ischemia in rats. / Hsiao, George; Fong, Tsorng H.; Tzu, Nien H.; Lin, Kuan H.; Chou, Duen S.; Sheu, Joen R.

In: In Vivo, Vol. 18, No. 3, 05.2004, p. 351-356.

Research output: Contribution to journalArticle

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