A pilot study for circadian gene disturbance in dementia patients

Hsing Cheng Liu, Chaur Jong Hu, Yun Chin Tang, Jan Gowth Chang

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Disturbance of circadian gene regulation might contribute to behavioral and psychological symptoms in dementia patients. This study was to evaluate the CpG island methylation status on the circadian gene promoters in dementia patients. We conducted a set of methylation specific polymerase chain reaction (mPCR) followed by nucleotide sequencing to analyze the methylation status within the promoters of nine circadian-related genes, including PER1, PER2, PER3, CRY1, CRY2, CLOCK, BMAL1, TIM and CK1ε, in the genomic DNA from the peripheral blood leukocytes of 80 dementia patients and 80 age- and gender-matched controls. A total of seven dementia patients (7/80) had CpG island methylation in the circadian genes and none of the controls had methylation. There were three and four patients had CpG island methylation on the promoters of PER1 and CRY1, respectively. Dementia with Lewy body (DLB) patients had the significantly highest frequency of circadian gene CpG island methylation (35.7%). It suggested that epigenetic methylation of circadian gene was more prevalent in dementia patients, especially for the DLB patients. The significance of circadian gene methylation in clinical behavior/sleep disturbance in dementia patients needs further study.

Original languageEnglish
Pages (from-to)229-233
Number of pages5
JournalNeuroscience Letters
Volume435
Issue number3
DOIs
Publication statusPublished - Apr 25 2008

Fingerprint

Methylation
Dementia
CpG Islands
Genes
Lewy Body Disease
Behavioral Symptoms
Gene Frequency
Epigenomics
Sleep
Leukocytes
Nucleotides
Psychology
Polymerase Chain Reaction
DNA

Keywords

  • Alzheimer's disease
  • Circadian rhythm
  • Dementia with Lewy body
  • Epigenetic
  • Methylation

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

A pilot study for circadian gene disturbance in dementia patients. / Liu, Hsing Cheng; Hu, Chaur Jong; Tang, Yun Chin; Chang, Jan Gowth.

In: Neuroscience Letters, Vol. 435, No. 3, 25.04.2008, p. 229-233.

Research output: Contribution to journalArticle

Liu, Hsing Cheng ; Hu, Chaur Jong ; Tang, Yun Chin ; Chang, Jan Gowth. / A pilot study for circadian gene disturbance in dementia patients. In: Neuroscience Letters. 2008 ; Vol. 435, No. 3. pp. 229-233.
@article{fe2913f76d864309ae57dc48fc31c1b7,
title = "A pilot study for circadian gene disturbance in dementia patients",
abstract = "Disturbance of circadian gene regulation might contribute to behavioral and psychological symptoms in dementia patients. This study was to evaluate the CpG island methylation status on the circadian gene promoters in dementia patients. We conducted a set of methylation specific polymerase chain reaction (mPCR) followed by nucleotide sequencing to analyze the methylation status within the promoters of nine circadian-related genes, including PER1, PER2, PER3, CRY1, CRY2, CLOCK, BMAL1, TIM and CK1ε, in the genomic DNA from the peripheral blood leukocytes of 80 dementia patients and 80 age- and gender-matched controls. A total of seven dementia patients (7/80) had CpG island methylation in the circadian genes and none of the controls had methylation. There were three and four patients had CpG island methylation on the promoters of PER1 and CRY1, respectively. Dementia with Lewy body (DLB) patients had the significantly highest frequency of circadian gene CpG island methylation (35.7{\%}). It suggested that epigenetic methylation of circadian gene was more prevalent in dementia patients, especially for the DLB patients. The significance of circadian gene methylation in clinical behavior/sleep disturbance in dementia patients needs further study.",
keywords = "Alzheimer's disease, Circadian rhythm, Dementia with Lewy body, Epigenetic, Methylation",
author = "Liu, {Hsing Cheng} and Hu, {Chaur Jong} and Tang, {Yun Chin} and Chang, {Jan Gowth}",
year = "2008",
month = "4",
day = "25",
doi = "10.1016/j.neulet.2008.02.041",
language = "English",
volume = "435",
pages = "229--233",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - A pilot study for circadian gene disturbance in dementia patients

AU - Liu, Hsing Cheng

AU - Hu, Chaur Jong

AU - Tang, Yun Chin

AU - Chang, Jan Gowth

PY - 2008/4/25

Y1 - 2008/4/25

N2 - Disturbance of circadian gene regulation might contribute to behavioral and psychological symptoms in dementia patients. This study was to evaluate the CpG island methylation status on the circadian gene promoters in dementia patients. We conducted a set of methylation specific polymerase chain reaction (mPCR) followed by nucleotide sequencing to analyze the methylation status within the promoters of nine circadian-related genes, including PER1, PER2, PER3, CRY1, CRY2, CLOCK, BMAL1, TIM and CK1ε, in the genomic DNA from the peripheral blood leukocytes of 80 dementia patients and 80 age- and gender-matched controls. A total of seven dementia patients (7/80) had CpG island methylation in the circadian genes and none of the controls had methylation. There were three and four patients had CpG island methylation on the promoters of PER1 and CRY1, respectively. Dementia with Lewy body (DLB) patients had the significantly highest frequency of circadian gene CpG island methylation (35.7%). It suggested that epigenetic methylation of circadian gene was more prevalent in dementia patients, especially for the DLB patients. The significance of circadian gene methylation in clinical behavior/sleep disturbance in dementia patients needs further study.

AB - Disturbance of circadian gene regulation might contribute to behavioral and psychological symptoms in dementia patients. This study was to evaluate the CpG island methylation status on the circadian gene promoters in dementia patients. We conducted a set of methylation specific polymerase chain reaction (mPCR) followed by nucleotide sequencing to analyze the methylation status within the promoters of nine circadian-related genes, including PER1, PER2, PER3, CRY1, CRY2, CLOCK, BMAL1, TIM and CK1ε, in the genomic DNA from the peripheral blood leukocytes of 80 dementia patients and 80 age- and gender-matched controls. A total of seven dementia patients (7/80) had CpG island methylation in the circadian genes and none of the controls had methylation. There were three and four patients had CpG island methylation on the promoters of PER1 and CRY1, respectively. Dementia with Lewy body (DLB) patients had the significantly highest frequency of circadian gene CpG island methylation (35.7%). It suggested that epigenetic methylation of circadian gene was more prevalent in dementia patients, especially for the DLB patients. The significance of circadian gene methylation in clinical behavior/sleep disturbance in dementia patients needs further study.

KW - Alzheimer's disease

KW - Circadian rhythm

KW - Dementia with Lewy body

KW - Epigenetic

KW - Methylation

UR - http://www.scopus.com/inward/record.url?scp=41549090375&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=41549090375&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2008.02.041

DO - 10.1016/j.neulet.2008.02.041

M3 - Article

C2 - 18358604

AN - SCOPUS:41549090375

VL - 435

SP - 229

EP - 233

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -