A novel zinc finger protein 219-like (ZNF219L) is involved in the regulation of collagen type 2 alpha 1a (col2a1a) gene expression in zebrafish notochord

Huang Wei Lien, Chung Hsiang Yang, Chia Hsiung Cheng, Chin Chun Hung, Wei Hao Liao, Pung Pung Hwang, Yu San Han, Chang Jen Huang

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The notochord is required for body plan patterning in vertebrates, and defects in notochord development during embryogenesis can lead to diseases affecting the adult. It is therefore important to elucidate the gene regulatory mechanism underlying notochord formation. In this study, we cloned the zebrafish zinc finger 219-like (ZNF219L) based on mammalian ZNF219, which contains nine C2H2-type zinc finger domains. Through whole-mount in situ hybridization, we found that znf219L mRNA is mainly expressed in the zebrafish midbrain-hindbrain boundary, hindbrain, and notochord during development. The znf219L morpholino knockdown caused partial abnormal notochord phenotype and reduced expression of endogenous col2a1a in the notochord specifically. In addition, ZNF219L could recognize binding sites with GGGGG motifs and trigger augmented activity of the col2a1a promoter in a luciferase assay. Furthermore, in vitro binding experiments revealed that ZNF219L recognizes the GGGGG motifs in the promoter region of the zebrafish col2a1a gene through its sixth and ninth zinc finger domains. Taken together, our results reveal that ZNF219L is involved in regulating the expression of col2a1a in zebrafish notochord specifically.

Original languageEnglish
Pages (from-to)872-886
Number of pages15
JournalInternational Journal of Biological Sciences
Volume9
Issue number9
DOIs
Publication statusPublished - Sep 5 2013

Fingerprint

Notochord
Collagen Type II
collagen
zinc finger motif
Zinc Fingers
Zebrafish
Danio rerio
gene expression
zinc
Gene Expression
protein
Proteins
proteins
Rhombencephalon
brain
promoter regions
Body Patterning
gene
Morpholinos
luciferase

Keywords

  • Collagen type 2 alpha 1a
  • Notochord
  • Transcriptional regulation
  • Zebrafish
  • Zinc finger protein 219

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Biology
  • Ecology, Evolution, Behavior and Systematics
  • Applied Microbiology and Biotechnology

Cite this

A novel zinc finger protein 219-like (ZNF219L) is involved in the regulation of collagen type 2 alpha 1a (col2a1a) gene expression in zebrafish notochord. / Lien, Huang Wei; Yang, Chung Hsiang; Cheng, Chia Hsiung; Hung, Chin Chun; Liao, Wei Hao; Hwang, Pung Pung; Han, Yu San; Huang, Chang Jen.

In: International Journal of Biological Sciences, Vol. 9, No. 9, 05.09.2013, p. 872-886.

Research output: Contribution to journalArticle

Lien, Huang Wei ; Yang, Chung Hsiang ; Cheng, Chia Hsiung ; Hung, Chin Chun ; Liao, Wei Hao ; Hwang, Pung Pung ; Han, Yu San ; Huang, Chang Jen. / A novel zinc finger protein 219-like (ZNF219L) is involved in the regulation of collagen type 2 alpha 1a (col2a1a) gene expression in zebrafish notochord. In: International Journal of Biological Sciences. 2013 ; Vol. 9, No. 9. pp. 872-886.
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AU - Han, Yu San

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